Preparation And Evaluation Of Collagen Polypeptide And Shengji Yuhong Gel

Objective: To study an ideal preparation of Traditional Chinese Medicine for wound healing. Shengjiyuhong Gel was selected to explore its extracting process, qulity standards, tentative stability and pharmacology. Methods: The extraction condition of collagen polypeptide from collagen were established with the extraction transfer rate of it by orthogonality test; The extraction and purification condition of Radix Angelica Sinensis and Glycyrrhiza uralensis were established with the anointed rate and total flavonoids by orthogonality test; The extraction condition of Lithosper -mum erythrorhizon and Angelica dahurica were established with the extraction transfer rate of Shikonin and Imperatorin by orthogonality test;The preparation process was established with the shaping,munite quality and stability of the gel; Dracorhodin and Hydroxyproline were measured respectively by HPLC; And stability experiments and the study of the stimulus experiments and healing skin injuries on rats were carried out.Results: collagen polypeptide was zymolysised at 45℃for 6h, with the dosage of trypsin was 8% of the weight of collagen,80 times NaOH solution(pH=8); Radix Angelica Sinensis and Glycyrrhiza uralensis were extracted and refined by water and deposited by alcohol, Water extraction twice (10times,8times) ,1h each time; Lithospermum erythrorhizon and Angelica dahurica were extracted and refined by water 80%alcohol twice(8times,6times) ,1h each time; though experiment research, Carbomer-980NF was optimized as excipient,the concen -tration of Carbomer-980NF is 2%,the proportion of glycerol was 5%,polysor -bate-80 was 1%; The method of TLC and HPLC were reasonable and controllable, the purity of Dracorhodin was no less than 0.020% and the purity of Hydroxyproline was no less than 0.45%; 12 months stability test shows each index comply with the requirements.Conclutions: The preparation process of the gel was steady; the quality of the gel can be controlled and the determination methods can be reproduced; the effect of sponge for healing skin injuries on rats was significant difference from that of blank group.