Preliminary Studies On The Fermented Feed And Cross Breeding Of Tenebrio Molitor L.

The effects of several factors such as temperature, density, the turning point of growth velocity, feed moisture and fermented feed on the growth and population amounts of Tenebrio molitor were studied. And the effects of applying cross and radiation technique on the population descendants and the significance of choose breed were researched. The basic method of extracting protein from the larvae of Tenebrio molitor were studied in this paper. During the temperature scope from 20℃ to 32℃, the each instar duration was shortened along with the temperature's heightening, and instar amounts were decreased. But at 35℃, the larvae grew very slowly and the growth duration lengthened. There were two stages of the effects of density on the larval growth of Tenebrio molitor. The first stage from hatching to growing in 30 days, the average larval weight of high density was more than that of low density, the second stage, the larvae of low density grew quickly, and their weight added quickly. The larval weight gain was similar of middle density. So the high density in 0~30days and then the density of 4.7~7.9 one/cm2 in industrialized production should be adopted.In the different stage of the larval growth of T. molitor, the growth velocity wasn't identical. The larval weight added slowly in the first month after hatched, but in the following days, it added quickly. Under the condition of low temperature (15℃), the turning point of growth velocity appeared at about 60d. In the suitable growth temperature scope (20℃~32℃) of T. molitor, the larvae grew more quickly, their weight added greater and the turning point of growth velocity appeared earlier along with the temperature increasing, at 20℃, it appeared in 84 d; at 25℃, 74 d; at 30℃, 56,60 d; at 32℃, 70 d. The effects of different moisture content feed on the larval growth, which was that larval weight added greatly along with the moisture content increasing of feed, and the larvae grew quickly, their weight added greatly, but their mortality is high of the high moisture content (30%~35%) feed. In the contrary, the larvae grew slowly and the growth cycle was long. So the suitable moisture content (15~20%) feed was helpful to the larval growth and keep high population amount.The acidification, basification and enzyme treatment were used to disposethe corn halm powder, peanut vine powder , pachyrhizus vine powder and then fermented, to feed the little and middle instar larvae. The effects of feeding middle instar larvae were better than those of little instar larvae. The little larvae grew slowly feeding on fermented feeds, the difference was marked compared with the wheat bran. The middle instar larvae grew well, the difference was not marked compared with the wheat bran. Between different fermented feeds, the feeding effects of the peanut vine powder and pachyrhizus vine powder were better than that of the corn halm powder; comparing different treatment groups of the same kind of fermented feed, the feeding effects of the enzyme and basification treatment were better than that of the acidification treatment.In the cross descendants of Tenebrio molitor and Tenebrio obscurus, the character manifestation of the cross descendants were similar to the yellow mealworm mostly. The F1 generation individual was larger and grew more quickly than the normal one. Then we could compare the different character manifestation individuals of the descendants, to choose the fine individual and reserve the breed in time, so that transmissibility of these variation individuals could be stabilized. The genome DNA preparation method of T. molitor and T. obscurus was researched, which was as the base for identifying T. molitor and T. obscurus and their cross descendants using the RAPD (the random amplified polymorphic DNA) technique in the future. The radiated technique was used to dispose the eggs of T. molitor. The most of eggs couldn't hatch of the high dose treatment－the 1,2 treatment, the fewness hatched larvae couldn't grew normally. The e...