Studies On Matched Techniques Of Industrialization Of Valuable Orchids

Based on tissue culture.the paper have reseached the fertilization of orchids to direct the Industrialization.The main results are as follows:1.After being sterilized on the surface of explants, side-buds on pedicel of Phalaenopsis were inoculated into the startup culture medium.The result indicated that being sterilized with 75% alcohol (3 seconds)and 0.1% HgC12 (10minutes),the survival rate of explants were improved obviously.2.AC (Active charcoal) was believed the efficient method to prevent the account of browny explants.In experiment,different proportion of AC( 0.3%,0.5%, 0.8%,1%,1.2%)were used.It was advised that 0.8%AC was the best account to prevent browny.3.MS, Kyoto and VW were used to induce sprouts from side-buds on pedicel of Phalaenopsis,and 6-BA, NAA were used as plant-growth regulators. It showed that the culture medium Kyoto and Ms had the same effect in inducing sprouts. And MS+6-BA5mg/L+NAA0.1 mg/L was advised to be the ideal culture medium.4.Different Continious cycles (15d, 20d, 25d, 30d) were compared in the experiment of multiplication of clustered buds.It was found that the shorter the continious-cycle was,the higher the multiplication rate was. When the continious-cycle is long enough,the sprouts would become fade at last.5. Different natural-pickups were introduced into MS to enhance multiplication rate.The result indicated that CM had a little effect in enhancing multiplication rate,but BN obviously had the opposite effect,namely restrain.6.In order to study the differentiation ability of leaf of Phalaenopsis, and the producting-rate of PLB,leaves of different sequence were inoculated into suitable culture medium.It showed that the younger the leaf was,the better the differentiation ability was.7.Using different concentration of 6-BA to induce PLB from young leaf of Phalaenopsis,found that the denser the 6-BA was,the better the effect was.And 6-BA5mg/L was found the best concentration.8. Different Plant-Growth-Regulators combinations were used to induce the potential buds on stem of Dendrobium.lt was found that the potential buds of Dendrobium cound be induced easily,and that 6-BA2+NAA0.1 was the best combination after 60 days' culture.9.Experiment showed that strong light and appreciably high temperature made for big but feeble and few buds.On the contrary, faint light and appreciably lowtemperature made for many and strong buds.10. Effect of media (four type) and NPK fertilizer method on growth and flowering of Phalaenopsis were studied in plastics shed with method of orthogonal design and Gray relational grade analysis(complex index of evaluation).Results showed that media and fertilizer were the two dominating factors which influnce number of flowers. Treating combination of N(in NO3)200 (mg/L), with NPK(20%:15%:15%) were applied on sphagnum media by which the maximum gray relational grade was obtain before budding PK and trace elements were applied.Next is saw dust mix river snad (1:1) ,NO3 200 mg/L,N,P and K in proportion of 20%:10%:10%,NP and trace elements were fertilized before flower-bud breaking.The third was peanut hull amend with sand ,and N100mg/L in ratio of NPK(15%:15%:15%).The lowesd was that river sand amend KD-1-Hydrophilic gel.