Studies On The Pollen Character,Pollination Method And The Cultivation Of The Hybrid Of Cut Flower Lilies

In this experiment, Lilium Brownii var. viridulwn, Shennong 157 and 17 cultivars of cut flower lilies imported from Holland were employed as the experimental materials to carry on the studies on the pollen character, pollination method, and the cultivation of the hybrid of cut flower lilies.The optimum pollen culture solution and coloring agent for the lily pollen viability test, the effect of different storage condition on the viability and storability of lily pollen, the effect of pollination method on the seed formation after cross pollination and the effect of different cultivation method on the germination of the hybrids were explored through serial experiments. Hybrids were obtained from 18 parental combinations in Oriental hybrids and 5 parental combinations in Asiatic hybrids. Hybrids were also obtained in reciprocal cross between Shennong 157 and Lilium Brownii var. viridulum, and the optimum rooting culture medium was found after rooting trial on these hybrid seedlings by tissue culture.The results indicate that: the optimum concentration of sucrose for the germination of lily pollen is 100g/L, and boron can promote the germination of lily pollen greatly, with the optimum concentration 20mg/L. The effect of calcium is a little smaller than that of boron. The optimal culture solution is sucrose 100g/L+H3BO320mg/L+CaCl210mg/L, with the germination rate 44.59%. The optimum coloring agent is TTC with the dyeing time 25-30min.In the experiment of pollen storage , pollen collected in different flower growth period was treated under normal temperature, normal temperature and dry, 0℃-4℃, 0℃-4℃ and dry, -18℃-0℃, -18℃-0℃ and dry conditions. The results show that 0℃-4℃ and dry condition is the best storage condition, which can make the life-span of the pollen last for 45 days. Under the normaltemperature condition, the life-span of the pollen last for about 15 days.Conventional pollination, pollination in different flower growth period, pollination after the treatments of pollen culture solution BA, NAA, salt solution, cut-style pollination and the injection of pollen into the style were used in the experiment. The results show that the parental combination, flower growth period and the pollination method can all affect the seed formation.The results of hybrid embryo-culture and hybrid seed sowing showed that the suitable embryo age for in vitro culture is 81d-90d. The resting period of hybrid seeds from Oriental hybrids is longer than that of the hybrid seeds from Asiatic hybrids. It's good for the germination of the hybrid seeds from Oriental hybrids to soak them in the GA3 solution with the concentration 100g/L, while water soaking is better when it comes to the hybrid seeds from Asiatic hybrids. Among the hybrid seeds, there are many seeds that have no embryos.In the rooting experiment that carried on the hybrid seedlings cultivated by tissue culture from Lilium Brownii var. viridulumXShermong 157 and Shennong 157 X Lilium Brownii var. viridulum, the optimum rooting culture medium is MS +IBA2+AC1.5.