Identification Of Wheat-Thinopyrum Intermedium Translocation Lines Conferring Resistance To Barley Yellow Dwarf Virus By Cytology And Molecular Markers

Cross was made between wheat-Thinopyrum intermedium substitution lines and wheat cultivar Yangmai 10, and the immature embryos were cultured and the regenerated plants were induced to introgress the BYDV-resistant gene from alien substitution lines to wheat. BYDV resistance screening and cytology analysis were carried on among the resistance of regenerated plant offsprings and to get translocation lines and screen molecular markers that could track the BYDV-resistant gene. The BYDV-resistant materal will be used in the wheat breeding program, and molecular markers would facilitate the translocation lines with BYDV resistance screening effectively.i. Through genomic in situ hybridization (GISH) with Thinopyrum intermedium (E₁E₂St) genomic DNA as probe and Triticum astivum (ABD )genomic DNA as a blocker, it is demonstrated that the two lines 04Y5579-2 and 04Y5579-3 are translocation lines,which contain a Robertson translocation chromosome between wheat and Thinopyrum intermedium . Using rye genomic DNA as probe ,the result of GISH analysis showed that , translocation lines 04Y5579-2 contains rye 1RS chromosome.ii. In order to determine which wheat chromosome arms are absent in the two translocation lines 04Y5579-2 and 04Y5579-3,two translocation lines and their parents were used as the material and analyzed by 12 pairs SSR primers located at the wheat homologous group 2.The result showed that, all wheat microsatellite primer pairs located at the wheat 2A 2B and...