| Objective:To analyze the gene and protein expression levels of the Talin1and MMP (matrixmetalloproteinase)-2in gastric carcinoma tissue and the relationship of the gene andprotein expression level of Talin1and MMP-2and the hepatic metastasis of gastriccarcinoma was also been estimated. The effect of the cell proliferation and livermetastasis of gastric carcinoma on the gene and protein expression level of Talin1andMMP-2would be confirmed. The research would provide the clinical evidence to theearly predication, early intervention and prognosis improving in the early stage ofgastric carcinoma.Methods:A total of108patients with gastric carcinoma received operation in general surgerydepartment from January2008to December2012were chosen to research object. Themale patients were65cases and female were43cases. And the age range in allpatients was from37years to68years and the mean age was (52.8±13.6) years. Theselection criteria’s of this research were the below items:①The electro gastroscopicaland surgical specimens were pathological diagnosis before and after operation, andwere confirmed to adenocarcinoma.②The tumor lesion was never received bychemotherapy or radiotherapy before operation.All patients were divided into two groups, metastasis group (51cases, gastriccarcinoma had liver micro metastasis) and non-metastasis group (57cases, gastriccarcinoma hadn’t liver micro metastasis), according to liver micro metastasiscondition.(1) The analysis of normal clinical baseline indexes. The differences of normalclinical baseline indexes were compared between two groups, including age, genderratio, body mass index (BMI), tumor volume, tumor location (cardia-fundus, body,antrum and pylorus), invasion depth (T1, T2, T3and T4), pathological differentiateddegree (Borrmann type), histological differentiation degree, lymph node metastasis(N0, N1, N2and N3), peritoneum invasion (P0and P1) and vascular invasion, etc.The indexes which were different between two groups in the above11indexes wereconfirmed to risk factors on the liver metastasis of gastric carcinoma by multivariateLogistic regression analysis. (2) The analysis of the gene expression level of Talin1and MMP-2. The expressionlevel of CEA, AFP, VEGF, Talin1and MMP-2mRNA in gastric carcinoma specimentissue was detected by reverse transcriptase-polymerase chain reaction (RT-PCR)method. The relationships of the above11indexes to liver metastasis of gastriccarcinoma were analyzed by Pearson linear correlation analysis. The risk factors toliver metastasis of gastric carcinoma were analyzed by multivariate Logisticregression analysis from the above11indexes.(3) The analysis of the protein expression level of Talin1and MMP-2. Theexpression level of CEA, AFP, VEGF, Talin1and MMP-2protein in gastriccarcinoma specimen tissue was detected by immunohistochemistry S-P method. Therelationships of the above11indexes to liver metastasis of gastric carcinoma wereanalyzed by Pearson linear correlation analysis. The risk factors to liver metastasis ofgastric carcinoma were analyzed by multivariate Logistic regression analysis from theabove11indexes.(4)The predictive value of the associate detection of Talin1and MMP-2expression in the liver metastases of gastric cancer. The expression of Talin1andMMP-2were detected in patients with gastric cancer tumor samples by the method ofsingle-index method, parallel testing and test series, in order to evaluate the diagnostictest.Results:(1) The comparison of normal clinical baseline indexes. Compared tonon-metastasis group, the indexes of age, gender ratio, body mass index (BMI), tumorvolume, tumor location, invasion depth in metastasis group were no different (allP>0.05), but the indexes of pathological differentiated degree (Borrmann type),histological differentiation degree, lymph node metastasis (N0, N1, N2and N3),peritoneum invasion (P0and P1) and vascular invasion in metastasis group werehigher (all P<0.05). The indexes of pathological differentiated degree (Borrmanntype), histological differentiation degree, lymph node metastasis (N0, N1, N2and N3)and vascular invasion were confirmed to risk factors of liver metastasis of gastriccarcinoma by multivariate Logistic regression analysis.(2) The analysis of the gene expression level of Talin1and MMP-2. Compared tonon-metastasis group, the gene expression level of VEGT, Talin1and MMP-2mRNAin metastasis group were higher (all P<0.05), but the CEA and AFP mRNA inmetastasis group were no different (all P>0.05). The positive relationships of the expression level of VEGT, Talin1and MMP-2mRNA to liver metastasis of gastriccarcinoma by pearson linear correlation analysis. And the expression level of VEGT,Talin1and MMP-2mRNA were also confirmed to risk factors of liver metastasis ofgastric carcinoma by multivariate Logistic regression analysis.(3) The analysis of the protein expression level of Talin1and MMP-2. Comparedto non-metastasis group, the expression level of AFP, VEGT, Talin1and MMP-2protein in metastasis group were higher (all P<0.05), but the protein expression levelof CEA in metastasis group were no different (all P>0.05). The positive relationshipsof the protein expression level of VEGT, Talin1and MMP-2to liver metastasis ofgastric carcinoma by Pearson linear correlation analysis. And the protein expressionlevels of VEGT, Talin1and MMP-2were also confirmed to risk factors of livermetastasis of gastric carcinoma by multivariate Logistic regression analysis.(4)The predictive value of the associate detection of Talin1and MMP-2expression in the liver metastases of gastric cancer. As the single indicator toforecast liver metastasis of gastric cancer, the sensitivity of Talin1was98.04%andspecificity was26.31%. The sensitivity of MMP-2was96.07%and specificity was28.07%. Combined the indicators of Talin1and MMP-2as paralleled parameters, thesensitivity to forecast liver metastasis of gastric cancer was84.31%and specificitywas3.51%. As the indicators were used for series test in the diagnosis, the sensitivitywas70.59%and specificity was96.49%.Conclusion:(1) The indexes of pathological differentiated degree (Borrmann type), histologicaldifferentiation degree, lymph node metastasis, peritoneum invasion and vascularinvasion were related to liver metastasis of gastric cancer. And the indexes ofpathological differentiated degree (Borrmann type), histological differentiation degree,lymph node metastasis and vascular invasion were confirmed to the risk factors toliver metastasis of gastric cancer.(2) The indexes of VEGT, Talin1and MMP-2were related to liver metastasis ofgastric cancer in patients with gastric cancer. And the indexes of VEGT, Talin1andMMP-2could be used for predicting the liver metastases of gastric cancer effectively.(3) As single indicator for forecasting liver metastasis of gastric cancer, the specificityof Talin1and MMP-2were not perfect, so the single indicator could not predict theoccurrence of liver metastasis of gastric cancer efficiently. Combined the two indexesof Talin1and MMP-2in parallel experiments, the specificity was poor, so the predict value of parallel experiment was also poor. Combined the two indexes of Talin1andMMP-2in series test was the efficient diagnositic method to liver metastasis of gastriccancer. |
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