Preparation Of Chitin Compound Sustained Release Scaffold And Its Biological Characteristics

Purpose:It is the emphasis and difficulty to manufacture excellent scaffold for bone tissue engineering research. This study was designed to investigate the application of slow-release bracket and biological characteristics of chitin production of bone tissue engineering. Provide an important basis for future experiments applied to build practical bone tissue engineering.Method:Chitin solution was mixed with sodium alginate solution, and then the mixture was added a certain quality of hydroxyapatite. We divided the mixture into two groups according to the different chitin solution mass fraction:scal (0%chitin), sca2(50%chitin). The surface structure and the pore size was observed under the Scanning electron microscopic. Then we calculated the porosity, degradation rate, water content and biomechanical properties.Results:Two groups of scaffold materials showed a multi-pore structure. The average pore size were121.2±12.6μm and213.3±27.3μm. The porosity were (90.53±1.62)%and (87.73±1.22)%. Statistical analysis showed that two groups of material porosity difference were statistically significant (P<0.05). The degradation rates of two groups of scaffold materials at six weeks were (59.12±1.93)%and (22.91±0.953)%. Statistical analysis showed that the degradation rate of the material differences between the two groups were statistically significant (P<0.05). Water content of two groups of scaffold materials were (95.52±1.17)%and (90.42±0.85)%. Statistical analysis showed that the water content of the material differences between the two groups were statistically significant (P<0.05). Biomechanical properties of the second group increased significantly.Conclusion:It could be seen from the experimental data that chitin could increase the pore size, improved stability to degradation and the biomechanical strength of materials. Therefore, chitin may has important research value in bone tissue engineering field.