Single Nucleotide Polymorphisms Of Chromosome 9p21 And Genetic Susceptibility To Coronary Heart Disease

Objective To investigate the association of rs4977574in chromosome9p21single nucleotide polymorphisms (SNPs) with coronary artery disease(CAD).Methods This study was conducted with a case-control design including541patients with CAD and155controls by coronary angiography. The rs4977574polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.Results (1) Baseline comparison:No significant difference of gender, age, smoking, alcohol consumption, history of hypertension, diabetes, FPG, TC, TG, HDL-C, LDL-C, systolic blood pressure, diastolic blood pressure, BMI in CAD group and control group(P>0.05)was detected.(2) Rs4977574genotype GQAG and AA frequency in CAD group and control group was30.5%,63.0%,6.5%;25.2%,67.7%,7.1%respectively; All the distribution of genotypes was in Hardy-Weinberg equilibrium(CAD group χ2=2.079, P=0.377; control group χ2=.660, P=0.436);Compared to the controls, the frequency of risk genotype GG is on the increase in CAD group.(3)The prevalence of G or A allele was62.1%,37.9%;59.35%,40.65%;there was not significantly different between CAD group and controls(CAD group χ2=2.060, P=0.094; control group χ2=1.940, P=0.260); the frequency of risk allele G is on the increase in CAD group compared to the controls, too.(4) No significant difference between GG,AG and AA genotype and the quantity of involved coronary artery in CAD group and control group(χ2=14.286, P=0.429).(5) The relationship between the genotype of CAD group and Gensini score:three sets of data were positively skewed distribution; data fluctuation AA> GG> AG; maximum, P75:AA> GG> AG; P50:AA=GG> AG; P25, minimum were equal among the three groups.Conclusion(1)Rs4977574single nucleotide polymorphisms were not associated with coronary artery disease, the risk allele G may not increase the risk of coronary artery disease in this population.(2) May be no correlation between the genotype and severity of coronary artery.(3) Compared to the controls, the frequency of risk genotype or allele is on the increase in CAD group.