| Adjustable effect magic effect on SD rats with type 2 diabetes and the impact on the transformation of vascular smooth muscle cell phenotype Domestic and international numerous studies show that DM vascular complications problems into asked a difficulty the majority of patients, give patients caused great mental burden and economic pressure.So for DM vascular complications of the treatment is particularly important.At present, traditional Chinese medicine in DM vascular complications has made certain curative effect, especially the delay gangrene, diabetic foot, etc, have outstanding performance, it shows that the traditional Chinese medicine in the aspect of DM prevention and treatment of vascular complications in great potential and broad prospects.Adjustable effect best, understand, on the side of the "active" or collaterals, activating blood and remove stasis, widely used in clinical practice.Share, salvia miltiorrhiza, peony, frankincense, myrrh SiYao can promoting blood circulation to remove blood stasis ramrod two focal waterways, is Chinese and western medicine combined with commonly used medicines for the treatment of vascular diseases. Studies show that the party in patients with diabetic nephropathy, can reduce blood BUN, SCr,24 hours urinary protein, whole blood viscosity and fibrinogen than the control group were improved significantly after treatment.This topic before the experiment also showed that the active effect of magic for inhibition of high-fat feeding rabbit atherosclerosis has also have a significant effect.In order to further explore active effect best in diabetic vascular disease research, the experimental group were further explored.This topic through the establishment of the rat model with 2 TDM, from the study of 2 TDM rats biochemical indexes and the VSMC phenotype transition mechanism to explore two aspects, the traditional Chinese medicine in the treatment of DM vascular complications of the role of a preliminary discussion.Research purposes:1, through active effect magic ACTS on the 2 TDM rats, intervention by Chinese medicine, blood sugar and glucose tolerance were observed before and after the experiment rats, the effect of weight, analysis of adjustable no 2 TDM to treatment effect of biology in rats. No role, through the active effect on 2 TDM rats, by Chinese medicine intervention, in type 2 diabetic rat VSMC TGF-beta/Smad4 signal express way as the breakthrough point, through the observation of VSMC phenotype transformation in the process of PDGF-BB and related marker protein SM22 alpha and OPN content to study magic adjustable mechanism for cardiovascular disease prevention and control of diabetes.The research methods 1,80 SD rats were divided into control group, model group, metformin group and low dose group, Chinese medicine Chinese traditional medicine dose group, high dose group, Chinese traditional medicine, five groups of high-fat feeding 8 weeks after intraperitoneal injection of low-dose chain urea drug preparation (STZ) in type 2 diabetic rats model, to drug lavage after 4 weeks, observe the general situation of rats, the dynamic monitoring each rat body weight, fasting blood sugar levels, and before the treatment,2 weeks and 4 weeks after the treatment of impaired glucose tolerance.2,80 SD rats were divided into control group, model group, aspirin group and low dose group, Chinese medicine Chinese traditional medicine dose group, high dose group, Chinese traditional medicine in the five groups of high-fat feeding after 8 weeks, intraperitoneal injection of low-dose chain urea toxin (STZ) preparation of type 2 diabetic rats model.No continuous lavage adjustable effect. In each group for 30 days to be put to death.In rat carotid artery section, HE staining to observe vascular smooth muscle cell pathological changes, real-time fluorescent quantitative PCR method to detect carotid PDGF-BB, SM22 alpha and Smad4 and OPN expression.The results of the study:1,4 weeks after the treatment, compared with model group, Chinese medicine can reduce diabetic rats fasting plasma glucose (p< 0.05), impaired glucose tolerance improved (p< 0.05), fasting blood glucose by western medicine group (p< 0.05), impaired glucose tolerance is improved (p< 0.05), weight loss (p< 0.05).Adjustable effect magic can alleviate diabetes contributes to weight loss, but has no obvious effects of relation.2, Compared with model group, Huoluoxiaoling Dan dose group only mild proliferation of vascular smooth muscle cells. PDGF-BB of model group was significantly higher than those of the normal group, compared with model group, the expression of PDGF-BB traditional Chinese medicine group in blood vessel decreased significantly (P<0.05).Conclusion:1, adjustable magic can improve type 2 diabetic rats fasting glucose and impaired glucose tolerance, each group curative effect dose>> western medicine group in order to low dose group> high dose group, showed that active effect magic have a positive effect on the treatment of type 2 diabetes.2, adjustable magic can effectively reduce the VSMC phenotype transformation, but also interfere with the TGF-beta/Smad4 signaling pathways, cut Smad4, raised SM22 alpha, at the same time have antagonism effect of PDGF-BB fell, the synthesis of phenotypic marker protein expression of OPN drops. |
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