| Objective To explore the exressions of BDNF, GDNF and Nogo-A in rat spinal cord tissues with acute spinal cord injury (ASCI), and then to study whether neuroserpin(NSP) could change these expressions to protect the neural function.Methods 130 SD rats were randomly divided into three groups:SCI group(60),NSP group(60) and control group(10). The ASCI models were made according to the Allen’s weight drop method. SCI group were injected with 25μ1 normal saline, and NSP group were treated with 25 μ 1 neuroserpin both by intrathecal injection immediately after ASCI. In Control group, we only removed the vertebral plate and did not injure the spinal cord. Basso Beattie Bresnahan(BBB) scores were used to evaluate the spina cord functional deficits. Western blot and realtime PCR were used to detect the expressions of BDNF, GDNF and Nogo-A.Results There were no significant difference in BBB scores between SCI group and NSP group. Western blot and Realtime PCR both indicated that GDNF expressions after ASCI increased compared with control group, significantly in Id group, and the GDNF expressions of NSP group were higher than these of SCI group in 1d,3d,7d,14d after ASCI respectively. Nogo-A expressions after ASCI increased compared with control group, significantly in 14d group, and NSP group was lower than SCI group in every time point respectively. Meanwhile, realtime PCR showed the BDNF expressions after ASCI had reached the highest in 1d group and were always higher than the control group. However, the BDNF expressions of NSP group were significantly lower than these of SCI group in 1d,3d,7d,14d after ASCI respectively.Conclusions The expressions of BDNF, GDNF and Nogo-A after ASCI all increased compared with control group. There were no significant improvement in the motor function of ASCI rats with neuroserpin by intrathecal injection, but neuroserpin could promote the neural function repairment after ASCI in the gene and protein level.
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