Study On The Regularity Of Primary Dysmenorrhea And Pharmacodynamics

Purpose:Over the past10years of Chinese medicine for the treatment of primarydysmenorrhea related reports and "" Puji side "database managementsystem" related disorders use comprehensive analysis of the situation,to reveal the primary dysmenorrhea syndrome differentiation and treatment.According to the characteristics of traditional Chinese medicinetraditional Chinese medicine compound prescription sieving theintervention of primary dysmenorrhea, and its effect is verified by animalexperiment. To provide scientific basis for clinical application and drugcandidateMaterials and methods：1Objective Analysis the syndromes of stroke and guide the clinicaltherapy1.1Using CNKI, Wanfang periodical and VIP database system nearly10yearsof clinical treatment of dysmenorrhea related literature, summed up thelaw of syndrome differentiation; use "" Puji side "database managementsystem" retrieval of primary dysmenorrhea and its related disordersancient medication, medication characteristics and rules of inductiveanalysis.1.2Through the use of rules and related symptoms and dysmenorrhea,ancient and modern medicine confirmed, commonly used drugs and drugs onthe similarities and differences. The diagnostic criteria of literatureand clinical reports, confirming the difference, dysmenorrhea syndromedifferentiation problems and potential differentiation trend, combinedwith the ancient and modern medication rule selected indications and drugcombination 2The experimental study of Shu Granule on experimental dysmenorrhea2.1Effect of Jingshu Granule on mice analgesia experiment：2.1.1The pain induced by hot plate method in mice: the female mice wereplaced in the adjustable constant temperature hot plate, temperature (55±0.5)℃, the mice showed licking foot movement required time as thepain threshold of the rats, mice were measured repeatedly normal painthreshold of2times,2times the average value is calculated, and thenrandomly divided for the6group, continuous intragastric administrationof7D (blank control group to distilled water),7d after administrationof30min hot plate analgesia experiment,30min and60min were measuredtwo times the pain threshold.2.1.2The mice pain induced by acetic acid in mice experiment: group andadministration, continuous administration of7D,1h after the lastadministration, ip2%glacial acetic acid0.2ml/, after administrationwith a stopwatch to observe and record the mice pain time (latency)writhing and within15min.2.2Effect of Jingshu Granule on oxytocin induced dysmenorrhea model ofpain:Mice daily subcutaneous injection of E22mg/kg, continuousadministration of12D (artificial cause animal in estrus, in order toimprove the drug sensitivity of uterine smooth muscle), from the first7d administered to each group administered14d, in the14d1H IP afterintragastric administration of oxytocin20μ/kg, record given oxytocinin mice emergence of writhing reaction time (writhing latency) and30minin writhing.2.3Effect of Jingshu Granule on the isolated rat uterus:Healthy female non pregnant SD rats60were randomly divided into6groups. The body weight of180-200g rats by subcutaneous injection of E2,1mg/kg,3d. The day of the experiment used a stick to rat out, quickly opened the abdominal cavity, gently peel uterine peripheral adiposetissue, clipping uterine about1cm, immediately placed in a Rockwellliquid oxygen Michael’s bath, temperature constant temperature water bathmaintained at (37±0.5)℃, end ligation and fixed on the L shapeventilation channel, on the other end and tension transducer connectedto a polygraph, uterine keep resting tension of1g, began to record normaluterine contraction curve stable after1h, and then the experimental drugdifferent joining Maxwell bath, bath final concentration is the drug dose.Observe and record the uterine contraction frequency, amplitude andactivity (frequency*amplitude) changes.2.4Jingshu Granule on oxytocin induced isolated rat uterine smooth musclecontraction body effectHealthy female non pregnant SD rats48were randomly divided into6groups，The body weight of180-200g rats by subcutaneous injection of E2,1mg/kg,3d. The day of the experiment used a stick to rat out, quicklyopened the abdominal cavity, gently peel uterine peripheral adiposetissue, clipping uterine about1cm, immediately placed in a Rockwellliquid oxygen Michael’s bath, temperature constant temperature water bathmaintained at (37±0.5)℃, end ligation and fixed on the L shapeventilation channel, on the other end and tension transducer connectedto a polygraph, uterine keep resting tension of1g, start shrinkage curves,log normal uterus in vitro stability after1h10min, adding oxytocin13.89μ/kg, the isolated uterine smooth muscle contraction was significantlyenhanced, about10min after oxytocin effect reached the peak, and basedon the stability of the10min level, and then select add different liquid,compared to the difference in drug group and blank group.Results：1.Drug laws and dysmenorrhea and related disordersIn the previous month water to commonly used drugs abdominal pain were angelica, chuanxiong rhizome, sweet scented osmanthus, tree peony bark,ginseng, Mu Xiang, Yan Husuo, peach kernel, GUI Xin; nearly ten yearsacademic circles of angelica, Rhizoma Chuanxiong, Rhizoma Cyperi iscommonly used, Radix Paeoniae Alba, Yan Husuo, cinnamon, trogopterus dung,licorice, small fennel, saffron, treatment of dysmenorrhea; Chineseangelica, Rhizoma Cyperi, often with Yan Husuo, white peony root,chuanxiong, safflower, motherwort, three seven, licorice root, Danshentreatment. The ancient and modern treatment of dysmenorrhea angelica,chuanxiong, Radix Paeoniae Alba and common Corydalis, Cyperus rotundus,widely used in modern.2.Dysmenorrhea differentiation standard and clinical dialecticaldifferences and problems"Traditional Chinese medicine new medicine clinical research guidingprinciple","standards of TCM syndrome diagnosis and treatment" and"Chinese gynecology dysmenorrhea syndrome differentiation" to havesimilarities and differences, excluding repeat, there are cold dampstagnation, qi stagnation and blood stasis, Hanning uterus, blood stasis,deficiency of Qi and blood, deficiency of liver and kidney two, kidneydeficiency, liver meridian, the damp heat and blood stasis, stagnationof the liver and11certificates, academic amplified to20cards, eachcard belonging to various symptoms, syndrome of qi stagnation and bloodstasis as many as28. Every syndrome dysmenorrhea and its symptoms seriousconfusion, dilution on the treatment of dysmenorrhea syndromedifferentiation characteristics.3.Effect of Jingshu granule mouse pain threshold induced by the hot plateThe Jingshu granule in low, high, group after administration of60minhas obvious analgesic effect, and after administration of30min than asignificant effect, effect of longer duration.4.Effect of Jingshu Granule on acetic acid induced pain in mice The chemical pain Jingshu granule in low, high, group of mice inducedby acetic acid and has good effect; the Jingshu granule, high effect, lowin the writhing times of the mice in a dose-dependent manner.5.Effect of Jingshu Granule on oxytocin induced dysmenorrheaThe writhing latency, Jingshu granule in low, high dose could prolongthe intraperitoneal injection of oxytocin induced dysmenorrhea, reducethe writhing times of the mice injected with palace especially within the30min, and with the dose increased the writhing times decreasedsignificantly, there were dose dependent.6.Effect of Jingshu Granule on the contraction of uterine smooth muscleof rats in vitroThe Jingshu granule of low, middle and high dose group, administeredbefore and after, can significantly reduce the contraction of isolateduterus of rat frequency, amplitude and activity, and increased withincreasing dose of three indicators decreased significantly, on ratisolated uterine contractions in a dose-dependent effect; the ratisolated uterus the contraction frequency effect of high dose group isbetter than the Yimu granules group.7.Effect of Jingshu Granule on the contraction of uterine smooth muscleinduced by oxytocin in SD ratsThe oxytocin induced contraction of isolated uterus of frequency,amplitude and activity, the Shu particles in each dosage group has verygood curative effect and good effect in Leonurus granule group, but nostatistical significance.Conclusions：1.Standardization of TCM dysmenorrhea, dysmenorrhea syndromedifferentiation should be a fundamental solution to the nationalstandards, industry standards and textbooks, academic and among variousstandards widespread confusion. Suggestions of dysmenorrhea syndrome differentiation should distinguish primary and secondary; in thespecification, first identify and merge on the same card with synonymssynonyms and disease; the necessary choice of virtual symptoms exist inlarge quantities and virtual card.2.Although the dysmenorrhea clinical syndrome is chaotic, but still cansee the stagnancy of Qi and blood stasis is dominant in tcm. The ancientprescription on blood stasis using tiaojingzhitong and Qi tiaojingzhitongall medicine, also confirmed this point. Therefore, in order to regulatingqi and activating blood circulation, regulate menstruation pain is thebasic treatment, qi stagnation and blood stasis type selection based onthe general prescription, grasped the fundamental theory of syndromedifferentiation treatment of dysmenorrhea.3.Jingshu granule for the pain caused by physical and chemical stimulationhas good inhibition, from the statistical data shows, there were dosedependent effect of pain threshold and the number of writhing in mice,the greater the dose effect is more obvious; Jingshu Granule on oxytocininduced large, mouse uterine contraction play a significant mitigationeffect of rat, direct effect on the contraction of isolated uterus offrequency, amplitude, activity effect. And listed species Yimu granulesthan, the Shu particles of high dose better. The Shu particles have moreprecise tiaojingzhitong effect, can be used as candidate drugs for thefollow-up research and development.