Study On The Chemical Constituents Of The Small Polar From Chinese

Aquilaria sinensis Gilg, a tropical and subtropical evergreen trees, belongs to the family Thymelaeaceae. Healthy white wood does not produce agarwood only by the injure of natural factors or human factors, agarwood will gradually formed in the white wood of these plants. The content of volatile oil of agarwooc is higher, with rich aroma, volatile properties is the main medicinal components of agarwood. In this paper, compounds about chinese agarwood of petrolum ether extract were isolated and the anti-cancer activity of isolated compounds was evaluated.By various chromatographic methods,16 compounds were isolated from the petroleum ether extract of Chinese agarwood, their structures were determin ed by various spectroscopic methods.The names of compounds are followed: 6-methoxy-7-hydroxy-2-(2-phenylethyl) chromone (1*),18-norpimaric acid-8 (14),15-dien-4a,7a-diol (2*),7a-hydroxypimarol (3*), 7-oxo-6a-hydroxylevopimaric acid (4*),6,7-dimethoxy-2-(phenylethyl) chromone (5),6,7-dimethoxy-2-[2-(4’-methoxyphenoxy) ethyl] chromone (6),6-methoxy-2-[2-(3’-methoxy-4’-hydroxyphenyl)ethyl] chromone (7), 18-nor-abietic acid-8,11,13-triene-4a,7a-diol (8),18-nor-abieta-4α,7β-diol (9),7α-hydroxydehydroabietic acid (10),2α-hydroxydehydroabietic acid (11), 2β-hydroxydehydroabietic acid (12),7α,15-dihydroxydehydroabi etate (13),7-oxo-13β-hydroxyabiet-8(14)-en-18-oic acid (14), 2β-hydroxypimaric acid (15),3β-hydroxypimarol (16). Among them, com poundsl*,5-7 are 2-(2-phenylethyl) chromones, compounds 2*-4*,8-16 are diterpenoids/nor-diterpenoids.Four new compounds were founded, which belonging to chromones, diterp enoids. Compound 1* is a 2-phenylethyl chromone, Compounds 2*、3*、4* are diterpenoids, Compounds 2*、3* are derivatives of pimaric acid, Compound 4* is a derivative of levopimaric acid. All diterpenoids/nor-diterpenoids compounds were first isolated from Chinese agarwood.The cytotoxicity of 16 isolated compounds against five kinds of human tumor lines was evaluated, unfortunately, no significant activity were detected at 10μM concentration.