Effects Of Garlicin On Proliferation And Proliferation Of Human Pancreatic Cancer Cell Line Capan - 1 In

[Objective]:To study the effect of Allicin treatment on cell proliferation, apoptosis, cell cycle and cell migration on human pancreatic cancer cell line Capan-1.[Methods]:We performed MTT assay to measure cell viabilities of Capan-1 cells treated at different Allicin concentrations and time intervals to determine the optimum Allicin treatment concentrations and time point. We also calculated the IC50 by SPPS according to MTT inhibition assay and examined the cell morphological changes under the microscope. We used flow cytometry to analyze cell cycle and cell apoptosis under Allicin treatment. At last, we used cell scratch test to investigate cell migration and invasion abilities.[Results]:We observed more than 95% Capan-1 cells were alived in-vitro culturing. The cells reached their logarithmic grow phase after 24 hours cell passage. The MTT test showed a time dose dependent inhibition effect of Allicin and the calculated IC50 is 10mg/L. Flow cytometry analysis suggested cell cycle arrested at G2/S period and there is an on going cell apoptosis after Allicin treatment. Finally, the cell scratch test showed that the Allicin prohibited cell migration.[Conclusion]:Our studies show that Allicin can prohibit Capan-1 cell proliferation in a does and time dependent manner. Allicin induces cell apoptosis and arrests cell cycle at G2/S period, which eventually inhibits cell proliferation. Allicin also reduces cell migration abilities. Therefore, we postulate that the Allicin treatment will be a potential drug in treating pancreas cancer.