| Objection:To explore the repairment of UC-MSCs for MRL/lpr mice inflammatory microenvironment in vivo by using male MRL/lpr lupus mice through UC-MSCs transplantation and detecting the expression of inflammatory markers, innate immune receptors and B cell function in MRL/lpr lupus mice.Methods:25 male MRL/lpr mice were divided into three groups, group C (control group, n=5), group UT1 (UC-MSCs injection once, n=10), and group UT3 (UC-MSCs injection three times, n=10). Mice in group C got intravenous injection of saline 0.3ml weekly at 16-week,17-week and 18-week old, mice in group UT1 got intravenous injection of UC-MSCs suspension, with the density of 5 × 105/0.3ml at 16-week old; group UT3 got intravenous injection of UC-MSCs suspension, with the density of 5 x 105/0.3ml at 16-week,17-week and 18-week old. Mouse orbital sinus blood collection were performed at 16-week,18-week,20-week,22-week,24-week, 26-week,28-week,30-week, blood volume was 300ul for each mouse each time. The blood serum was separated, which were used in the subsequent ELISA experiment. The expression of IFN-y in blood serum was detected by ELISA, and the level of CD19/CD86 of B cells was detected by FCM. Mice in group C at 30-week old were sacrificed for blood and organs collection; both mice in group UT1 and UT3 were sacrificed at 20-week and 30-week for blood and organs collection. The expression level of innate immune receptor TLR7 and B cell function receptor complex CD79a in spleen tissue was detected by qRT-PCR.Results:1. From 16 to 20 week, the expression of IFN-y in three groups showed upward trend. The expression of IFN-y in group C and group UT1 kept rising till 28 week, and then showed downward trend. The expression of IFN-y in group UT3 increased till 26 week, and then the decreased.2. In 20th week, the expression of TLR7 mRNA in spleen in group UT3 was lower than group UTl,and with significant (P<0.05). In 30th week, the expression of TLR7 mRNA in spleen in group UT3 and UT1 all increased, while the expression of TLR7 mRNA in spleen in group UT3 was lower than that of group UT1, and there was statistically significant (P<0.01).3. In 20th week, the expression of BCR receptor complex CD79a mRNA of spleen in group UT1 and group UT3 were different, the expression of CD79a mRNA in group UT3 was higher (P <0.05). In 30th week, the expression of CD79a mRNA in three groups showed no significant difference.4. The expression of CD19+ in peripheral blood lymphocytes of group C showed the trend of continuous rise, while in group UT1 and group UT3, the curves were very plane, and the three groups showed no significant difference. The expression of CD19+CD86+ in mice peripheral blood in three groups showed no significant difference.Conclusion:1. UC-MSCs can reduce IFN-y concentration in MRL/lpr mice and the expression of spleen TLR7 mRNA, and this effect may depend on treatment times of UC-MSCs.2. UC-MSCs can inhibit the proliferation of B lymphocytes and reduce the expression of BCR of B cells in MRL/lpr mice.3. UC-MSCs have no effect on the expression of B lymphocytes costimulatory factor (CD86) of MRL/lpr mice in vivo.
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