Preparation And Properties Of Tissue Engineering Support Based On Electro - Spinning Technology

Tissue engineering technology can effectively solve so many problems, such as various cause of tissue and organ defects, that has become the Gospel of majority patients. Electrostatic spinning technique, which can simulate nano-reticular structure of nature extracellular matrix, has been widely applied in the preparation and research of tissue engineering scaffolds. Konjac glucomannan (KGM) and Sodium Hyaluronate (SH) were used as raw material, by the means of electrostatic spinning, KGM fiber mat and KGM/SH fiber mat were obtained, the feasibility of their applied to cartilage regenelation were discussed finally.Through the early experiment exploration of KGM single spinning solvent, the spinning system of glycerol/ice acetic acid/water were turned to be rationality. The influence of spinning system and sol concentration to spinning ability were investigated deeply by the orthogonal pilot program L9 (33). Three well spinnability groups of KGM fiber mat were obtained, namely 2#, 5# and 8#. Adopting the method of layered electrostatic spinning,2# were selected as the carrier, compound on SH, whose volume concentration was 1.5% and represented well spinnability, KGM/SH composite fiber mat were prepared. Finally the two kinds of fiber mats were basic cross-linked and subsequently processed, stable performance of KGM fiber mat and KGM/SH composite fiber mat were got. The orthogonal experiment results show that as to the spinnability, compared with the solution concentration, the amount of alcohol/acid revealed more influential. Glycerol had the greatest influence on the viscosity of the spinning sol, Ice acetic acid followed, KGM content was minimum. Water absorption tests showed that, KGM fiber mat absorptivity was higher than 4000%, while KGM/SH composite fiber mat was higher than 7500%. Basic crosslinking tests showed that, the hydrophilic and water resistance of the fiber mats could be significantly improved by the means of basic crosslinking.XRD, FT-IR, TG-DSC, SEM and MTT assay were applied to characterize the fiber mats. The crystalline, chemical structure, thermal stability and KGM and SH interaction relationship were researched. The results were showed as follows: ①The crystallinity of fiber mat could be decreased by increasing ice acetic acid, while the addition of glycerol would improve the crystallinity, crosslinking could significantly improve the crystallinity as well. ②Part groups of the fiber mat turned blue shift, since the induced effects caused by high pressure during electrospinning process was greater than the hydrogen effect. By means of basic crosslinking could not only further make part groups turn blue shift, but also could remove the acetyl inside the fiber. ③The thermostability of fiber mats could be effectively improved by basic crosslinking. ④The morphology of KGM fiber mat was better, with the increase of concentration, fiber diameter distribution is scattered. KS fiber mat existed chemical tangles, the entanglement degree would be further improved by crosslinking treatment. ⑤When the concentration of leaching solution is less than 5.0 mg/ml, two kinds of fiber mat toxic effects was 0-1 level, has the good biological activities and cytotoxicity.