Detection Of Acute Leukemia Related Substances By Electrochemical DNA Sensor

Acute leukemia has done great harm to the children’s health. The classified and comprehensive diagnose is of great significance to the timely and effective treatment. From the genetic perspective, acute leukemia is caused by gene(such as p53 gene) mutations. These genetic mutations will cause changes in morphology and the molecular level of the cell and cause a large number of malignant cell proliferation. As the process of cancer occurrence and malignant development is mainly induced by the complex changes in molecular level, the sensitive and selective detection of molecular(such as lysozyme(Lys) and interferon gamma(IFN-γ)) of this level contribute to the understanding of the immunity of the patients and the classification of the disease, which is helpful in diagnosing.Electrochemical DNA biosensor has many advantages and is widely used in the detection of the sequence specific DNA and target proteins in recent years. Among the advantages, compatibility for miniaturization feature makes the electrochemical sensor an attractive candidate for two analytes testing systems. As the deepening of the research on signal amplification technology, the signal amplification strategy, which has the ability to remarkably improve the sensitivity of the assay, has been applied in analysis widely. In our work, based on the signal amplification strategy and the purpose of multi analytes simultaneous detection, we fabricated electrochemical DNA biosensors for acute leukemia related analytes(p53, Lys and IFN-γ) detection.On the basis of DNAzyme catalyzing, N.BstNB I-assisted target recycling strategy, AuNPs with large specific surface area and excellent catalytic ability, graphene(GR) with large specific surface area and excellent electron transfer ability, which amplified signal and promoted the detection sensitivity, a versatile label-free quadruple signal amplification biosensing platform for p53 gene ultrasensitive detection was proposed. The detection limit of this biosensor was down to 3.29×10-16 M. Furthermore, by the assisting of the excellent signal generation catalyzed by the DNAzyme, this method is exempt from conventional cumbersome labeling.On the basis of aptamer recognition and electrochemical “signal-on” and “signal-off” modes, a novel dual biosensor was established for the simultaneous determination of two analytes(IFN-γ and Lys) related to acute leukemia. The proposed biosensor can be easily fabricated and readily regenerated, accompanying with high sensitivity. Furthermore, this bosensor is resistant to the interferences and proved successful towards the practical analysis.