| Objective:1. To investigate the local acupoint hyperthermia treatment characteristics of Zanthoxylum bungeanum-cake-separated moxibustion(ZBCS-moxi) by measuring the local temperature changes and temperature curves of ZBCS-moxi on ST36 and BL23.2. To reveal the anti-inflammatory effect of ZBCS-moxi on rheumatoid arthritis (RA) rats.Methods:1. The research about hyperthermia treatment characteristics of ZBCS-moxi:(1) Forty health SD female rats were randomly divided into 4 groups (n=8/group):control group, model group, ZBCS-moxi group and Aconitum carmichaeli-caks-separated moxibustion group (ACCS-moxi group). RA was induced by injecting 0.1ml Freund’s complete adjuvant into the right hind paw of rats. Eleven days after CFA injection, the rats in ZBCS-moxi group received Zanthoxylum bungeanum-cake-separated moxibustion on "Shenshu"(BL23), "Zusanli"(ST36) acupoints and the dorsal right hind paw respectively for 18 times (once daily). The ACCS-moxi group was used as a positive control, in which the rats were treated with Aconitum carmichaeli-cake-separated moxibustion on the same selected acupoints and courses. At the first day of two courses, the local acupoint temperature changes and temperature-time curve on ST36 and BL23 were recorded. (2) Twenty healthy students were divided randomly into ZBCS-moxi group and ACCS-moxi group (n=10/group). Having been stimulated by moxibustion, initial heat-feeling temperature, comfortable temperature, tolerant temperature, initial pain-feeling time, tolerant pain-feeling time and the time of optimum temperature region of each group right Zusanli (ST36) were measured respectively with Temperature Recorder.2. The explore about anti-inflammatory effect of ZBCS-moxi:After a 3-week treatment, we investigated anti-inflammatory effect by measuring the changes of volumes of paws, observing the pathologic morphology of synovial membranes and detecting the concentration of IL-1β and TNF-a in serum.Results:1. The hyperthermia treatment characteristics of ZBCS-moxi:(1)the maximum and rising degree of surface temperature of "Shenshu" and "Zusanli" in ZBCS-moxi group (43.00±1.44℃,41.70±2.52℃)are distinctly higher than ACCS-moxi group (40.32±1.57℃,38.57±0.49℃) (P<0.05).In addition, the acupoint local temperature in pre-moxibustion (33.32±0.44℃,33.77±0.63 ℃)or post-moxibustion (35.28±0.24℃,35.10±0.30℃)of "Shenshu" are notably higher than the ones of "Zusanli", regardless of the experimental group or control group (P< 0.05). B. The initial heat-feeling temperature, comfortable temperature and tolerant temperature of both groups increased in proper order (P<0.05). The tolerant temperature of ZBCS-moxi group (49.92±2.77℃). was higher than that of ACCS-moxi group(47.21±2.70℃)(P< 0.05). The time of optimum temperature zone of ZBCS-moxi group (473±218.48s) was significantly longer than the one of ACCS-moxi group (235±201.34 s) (P<0.05).2. The explore about anti-inflammatory effect of ZBCS-moxi:Compared to model group, the swollen paw volumes(1.62±0.04ml,1.72±0.13ml)., the synovial hyperplasia and pannus formation of synovial membranes and the concentration of IL-1 β(120.49±4.23pg/ml,123.04±9.03pg/ml)andTNF-a (50.49±7.24pg/ml, 54.03±10.27pg/ml) of rats decreased obviously (P<0.01, P<0.05) in ZBCS-moxi group, as well as in ACCS-moxi group. All changes in both ZBCS-moxi and ACCS-moxi groups did not present significant differences (P>0.05).Conclusions:1. The thermal effect of ZBCS-moxi is remarkably higher than the one of ACCS-moxi. And, the thermal effect of different acupoints with the same moxibustion shows some difference. The threshold of heat sensitivity of ZBCS-moxi was higher than the one of ACCS-moxi, and the time of optimum temperature region of the former was longer than the latter’s, which provide experimental basis for the clinical application of ZBCS-moxi.2. ZBCS-moxi can give rise to anti-inflammatory effect on RA, which will be a part of evidences to explain how ZBCS-moxi works for RA rats. |
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