1. Cordycepin Enhances The Sensitivity Of A549 Cells To Cisplatin By Inhibiting NF-κB Pathway 2. The Culture Of Human Embryonic Stem Cells H9 And Its Growth Microenvironment On HepG2 Cells

ObjectiveTo observe changes of proliferation and apoptosis after the treatment of cordycepin (Cor) on human lung adenocarcinoma A549 cells in vitro. And then combined cordycepin with cisplatin(DDP), studying the anti-tumor effect of them. To investgate the effect of cordycepin or combination cordycepin with cisplatinon on the key protein NF-κBp65 in NF-κB signaling pathway and the downstream signaling molecules:Bax and Bcl-2. And to discuss its possible molecular mechanism of anti-lung cancer, in order to provide new ideas for clinical treatment of tumors.MethodsA549 cells were cultured in vitro and the cell proliferation was assessed by MTS assay after treatment of different concentrations of cordycepin or combination cordycepin with cisplatinon for 24 hours, Hoechst 33258 staining and Annexin V/PI flow cytometry were used to determine the apoptosis of A549 cells. The protein expression of nuclear factor-KBp65 (NF-κBp65), Bax and Bcl-2 was determined by Western blot.Result1. Cordycepin could obviously inhibit of the proliferation and promote the apoptosis of A549 cells. The Western blotting results showed Cordycepin could increase the expression of Bax and cleave Caspase-3, decrease the expression of Bcl-2. Compared with the control group, Cordycepin could inhibit the transference of NF-κBp65 in the cytoplasm to the nuclear.2. Cordycepin could enhance chemosensitivity of DDP on A549 cells. The results of Hoechst 33258 staining and flow cytometry indicated that the rate of A549 cells apoptosis inducted by the combination of DDP and cordycepin was obviously more than DDP alone. Compared with the control group, DDP made the expression levels of NF-KBp65 in A549 nucleus increasing, but cordycepin could decrease its expression, the combination of DDP and cordycepin could obviously reduce the indicators of NF-KBp65. The Western blotting results showed that Compared with the DDP alone group,the combination with cordycepin could significantly increase the expression of Bax and decrease the expression of Bcl-2.Conclusion1. Cordycepin could obviously inhibit of the proliferation and promote the apoptosis of A549 cells.2. Cordycepin could enhance chemosensitivity of cisplatin on A549 cells, the mechanism of action is achieved by inhibiting the NF-κB pathway, and regulating the expression of downstream signaling molecules:Bax and Bcl-2.