Detection Of HBV-DNA In The Colostra From Hepatitis B Virus Markers Seropositive Carrier Mothers By PCR

Objective: To investigate the safety of breast-feeding of hepatitis B virus markers( EBVM) seropositive carrier mothers, We detected HBV-DNA by polymerase chain reaction(PCR) in the colostra from mothers who were HBVM positive carrier. Methods: 62 HBVM seropositive carrier mothers and 10 normal controls were recruited. HBV-DNA was detected by PCR in the colostra. Results: The rate of HBV-DNA positive in the samples from mothers who were HBVM seropositive carrier was 56. 5%, HBV-DNA was not found in the control group. HBV-DNA was positive in 21 samples from 24 who were HBsAg (+), HBeAg (+) and Anti-HBc(+) (21/24); in 4 samples whose HBsAg(+) Anti-HBe(+} and Anti-HBc(+) (4/12); in 4 samples whose HBsAg(+)and HBeAg(+) (4/6) ; in 3 samples whose EBsAg(+) and Anti-HBc(+) (3/7); in 1 sample from 3 who were only HBeAg(+) (1/3); in 1 sample from 3 who were Anti-HBs(+) and Anti-HBc(+); in 1 sample from 3 who were Anti-HBe(+) and Anti-HBc(+). HBV-DNA was negative in the samples from mothers 2 who were Anti-HBs (+), Anti-HBc (+) and Anti-HBe (+). The positive rate in HBeAg(+) group was higher than that of HBeAg(-)group, there was a significant difference (P<0. 0001). Conclusion: HBV-DNA should be detected in the milk from mothers who were EBVM positive carrier, The infants born to mothers who were HBVM positive should be intensified immunoprotection. If HBV-DNA in the milk was positive, breast-feeding by an HBVM-positive mother should be discouraged.