Cloning And Sequence Analysis In Structural Protein VP1/VP2 Region Of Human Parvovirus B19

Human parvovirus B19 (PVB 19), the only parvovirus known to be pathogenic in human , belongs to the genus Parvovirus in the family Parvoviridae. It is the smallest animal DNA virus. PVB 19 infection often results in a variety of clinical manifestations in adults and children . The objective of this research was to preliminary study the variation of PVB19 in Guangdong Province. Firstly, we used Nest-PCR method to detect 496 serum samples obtained from 473 patients. 18 sampels obtained from 17 patients were positive for PVB 19 DNA. Of the 17 patients, two had clear clinical record. one was a child with acute lymphocytic leukemia, the other was a adult with chronic active hepatitis B associated with aplastic anemia .Then a methodology combined with re-PCR amplifying, TA cloning and nucleotide sequencing was used to analyses the 898bp fragment (2909-3806 nt) of the two strains Compared with standard strain, the rate of nucleotide variability of the two strains was very different, even the. two strains were isolated in Guangdong Province in the same time. We also compared the two strain抯 fragmentes with other associated strains抯 fragment, which was searched from Genbank . These data suggest that PVB 19 isolates from China were significantly different from other isolates at both the nucleotide and prediected amino acid levels, and that no particular genotype of PVB19 was associated with the particular clinical outcome.