The Expression Of Integrin α_vβ₃ And Insulin-like Growth Factor-I In Woman With Polycystic Ovarian Syndrome

Polycystic ovarian syndrome (PCOS) is the most common cause ofinfertility reason,there are about 50% to 70% aovulation infeftility fOrPCOS,Clomephene citrae is the first line of theraPy f9r ovulation induction ininfertile patients with PCOS. Howeveq neary l5% to 20% patients areresiStance to clomePhene citrate, these patiellts have to induction ovulation bygonadotropin, which easy lead to ovedan hyPershmulation syndrOIne(HOSS),To avoid severe HOSS,cryopreservation of all embryos for the transfer inan whficial cycle has also an effective altemative in PCOS patients at highriSk .Since PCOS patients in SPecial endocrine environment,how to aPPlyaPPropriate estrogen and progeSterone to prePare endometrium for betterrecePtivity is a problem to be resolved. domestic and intemational have nOtseen reported.Integhns family are transmembrane glycOProein Which make of twosubunits by no covalent bond, they express in endometrium periodically andsPecifically, integrin a.P3 aPPeedng on endometrium postovulatory days 5-6,corresPonding to the time of embryo attachment, The integrin a.63 has beeneXensively stUdied as a marker of endometrum recePtivity and is aberranlyexpressed in the some infertile woman endometrium, Integrin a.63 not exPressin endometrium of some with minimal and mild forms of endometriosis, hitealphase deficiency, communicating hydrosalpinx and unexplainableinfertility .the integrin a.03 appears to be an excellent marker to study themolecular events leading to the establishInent of uterine receptivity andsuccessful implantation. Insulin-like groed factor (IGF) is a kind ofmulti-funtion cell Proliferation regulation factor, synthesized in a vedety oftissues. IGFs can exert not only endocrine driction bat also mitogenic effectswithin the local tissue environment through auocrine andjr paracrinemechanisms. The lGF system expressing in human uterus, is believed toinvolve in proliferation and differentiation of the uterine endometrium duringthe menStrUal cycle, enhancement troPhoblan invasion, Promotion placentadevelopment, embryonic and fetal growth in human pregnancy, IGF-I mainlyexPress dwing proliferation Phase, pedicipate ce1l mitosis assistnd whestrogen .In this study we determinate the marker of endometrium recePivityinteghn as63 and IGF-I exPression on the endmetrium after aPplicatonprogesterone,analyzing the relation betwCen these matters and estrOgen orprogesterone for seek the best replace scheme for PCOS patienis.. We selected30 PCOS patients who following conditions in Zhejiang Obstetrics andGynecology hosPital from SePtember 2000 to March 200l, their basal bodyWatUre are single Phase and have nO use sex hOrmone for three months.At same time we selected 20 examples as controls, Whoes mensmiation arecontinuous regUlarly and basal body temPeratUr double Phase, without thehistory of having hOrmone aPPlication for three months. we emPloy 20mgProgesterone to PCOS patiClls everyday for seven days .patients: Collectserum before inject progesterone and after inject progesterone for seventimes,additionally,we respire endometrium from dridus of uterus withpippele .Control 'we collection sena in late fOllicular phase and implantationPhase, we also get endomeimim in the imPlanation phase, all materials storeat -80C ultralow temperature refrigerator We test serum E2. and P Wthe1ectric chIniluminescence and measure the thickness of endometrium byvaginal ultrasound,We used the SP immunohistochemistry techniques withmonoclonal anibodies specific to integrin av63' IGF- I' ER. PR to study theeXPression of integrin av63. IGF- l \ ER and PR in PCOS patiots andcontrols resPectively We observed the SP dyeing sections.under the CHKmodel olymPus microscope of 400 times. and Calculated semiquanitativeanalysis with the method ofhistology scoreResult:There were no differnce between two grouPs age, and the body massindex of patients were higher than of controls (P<0...