β-nerve Growth Factor Extracted From Brain And The Action Induced By Internal Contamination To Enriched Uranium(～(235)U)

Nerve growth factor (NGF) is a trophic factor required for survival growth development and differentiation of sympathetic neuron and sense neuron. It involves in the regulation of immune and neural interaction. Moreover, NGF may have therapeutic potential in the treatment of peripheral and central nervous system diseases such as Alzheimer's diseaseN neurodegenerative disease and peripheral nerve impairment. But because NGF is a protein with high molecular weight, its permeability through blood-brain barrier(BBB) is little and thus limits the application of clinical treatment. We considered that the weight of β-NGF, which is the main chain of NGF, is relative small and it may cross BBB partly, so we think it should have vast application value in clinic. In this report, we compared the extraction of β-NGF from fetal brain and SD rat brain first. With low-temperature and high-opeed centrifugation , ion exchange chromatography and dialysis , we separated and purified brain homogenate and got β-NGF. Then we tested the extracted β-NGF by neurite outgrowth test of PC12 cells and confirmed that it has high biological activity. Follow on, we study the action induced by enriched uranium (23W) on the main protein component of rat brain. We found that enriched uranium may lead to the reduction of the obtainable rate of β-NGF. It should be noted , that the reduction has a linear relation with uranium dose . Meanwhile we found that the action of enriched uranium may lead to cleavage of brain protein, thereby enlarge the range of molecular weight spectrum of main component and increase the quantity of protein band in SDS-AGE. The results can provide scientific basis for making protection standard and the evaluation of healthy protection during the application of nuclear energy. Postgraduate Hao Fu-Rong Directed by Acad. Zhu Shou-Peng Prof. Miao Jing-Cheng Prof. Zhang Lan-Sheng...