Collection,Seperatiing And Cryopreservation Of Blood Stem Cells

Background:Highdose chemotherapy with autologous hematopoietic stem cells support is now used widely in the treatment of metastatic breast caucer. This use of high-ose chemotherapy has been motivated by the results of uncontrolled , singlenstitution pilot studies that have demonstrated a high rate of conversion from partial remission to complete remission .This technique includes mobilization, collection, seperation, cryopreservation and recovery of HPC. In order to make highose chemetherapy with autologous hematopoietic cells support success, we will study cellection, seperation, cryopreservation and enumeration of HPC. Objective To study collection , processing and enumeration of peripheral blood heineatopoietic progeniter cells. Methods Ten cases of cord blood were collected by closed artery collecting method . Stem cells were seperated from cord blood by Ficoll?s solution. Three groups with 1O%DMSO, 5%DMSO?%HES?% Albumin (self prepared) and Cp? seperatly ,preserved in 96oC liquid nitrogen and ?OoC freezer. After cryopreserving, MHC in the samples were counted, CD34+ cells were enumerated by flow cytometry. 4 Results The volume of cellet ion was 72. 2 ?17. 3ml . The results showed no signficant difference among three groups of cryoprotectants in one , three and six month (p>0. 05). In the group (six month),the relative ratio of CD34?cells counts by flow cytometry was higher than that in other groups. within six months ,There are no significant difference between storing in ?OoC freezer and in ?96oC liquid nitrogen to stem cells (p>0.05). Conclusion Effect of cryopreserved solution (selfrepared) was good. Short period of cryopreserved could be safe in 80oC preezer. Stem cells cryopreserved in 1, 3, 6 months could be kept activation.