Detection Of Antibiotic-resistance And Comparison Of Genotyping Methods Of Proteus Bacilli

The susceptibility testing to 12 antibiotics of 32 clinical strains of Proteus mirabills and 12 clinical isolates of Proteus vulgaris which were isolated during 1998 to 1999 were performed by disk diffusion method suggested by current National Committee for Clinical Laboratory Standards (NCCLS) guidelines. The extended-spectrum P -lactamases (ESBLs) producers were tested by double-disk synergy test (DDST)Etest and confirmatory test of disk disfusion method. They were also studied by molecular biologic methods such as PCR PFGE and Isoelectric Focusing(IEF). The comparison study of four genotypic methods, Pulsed-field gel electrophoresis (PFGE),Random amplified polymorphic DNA (RAPD) Repetitive extragenic palindromic PCR (Rep-PCR) and enterobacterial repetitive intergenicconsensus PCR (ERIC-PCR), were performed to all the isolates. We found that all the isolates displayed high sensitivity to Imipenem (100%) which can be used as the best drug for ESBLs Proteus infectins. The sensitivity to Trimetoprim-sulfa,Ciprofloxacin and Gentamicin is low. All ESBLs producing isolates were multiple antibiotic-resistant. Cefotaxime was a more sensitive indicator than Ceftazime. The concordance of the methods used to screening ESBLs isolates were very well. DDST combined with the primary screen test of disk diffusion was a better method for its high sensitivity and specificity , low cost and easy to implement. The type of ESBLs can be preliminary assessed by PCR and Isoelectric focusing. PFGE is the best method in discriminating epidemic relative bacteria, but showing limitations in drug-resistant...