Studies On The Anti-tumor Constituents Of Cassia Siamea Lam

The stem of CaSho sboea colleCte from SiH in YUnnan Province, has beeninveStigated. Sixtee compounds were isolated. On the basis of spectral analysis (IR, EI-MS,FAB-MS, IHNMR, I3CNMR, DEPT lH-1H COSX HMQC, HMBC, NOESY,NOE-difference, CD et aI. ) and cheInical properties, six new compounds wer elucidatedtOghr with seven knOwn cOInpounds. These compounds are as fOllowin: 6 -sitOsterol(CS-1), betulic acid (CS-2), n-octacosanOl (CS-3), twsophanl (CS-4), ChrysoPhanl-1-O-6-D-glucOPyranside (CS-5), lupeol (CS-6), Pic--l (CS-7), 2-methyl--5wi -7, l2-dihydrWmone- l2- O- 6 -D-glucOPyranoside (C S - 8), l, l', 8, 8', l 09e~xyl-3, 3'--dimethl- l -O-a-D-glucOPynanSyl-7-C- 6 -D-glucOPyransyl- l 0' -C-9 -D-glucopyransyl- l 0,l0,-(9, 9')dianthrOn (CS-9), l - [(6 -D-glucoPyransyl-(l -6)-O- 9 -D-glucOPyransyl)oxy] - 8-hydroxy-3 -methy-9, l 0-antwuinOne(C S- l 0); l, 8, l 0-trihydrxyl-3 -Inethl- l 0-C(R)- p o-glucopHsyl-9-Wne (3 -desox yl- l 0- hydroxyoin A) (CS - 1 l ); 1, 8, 1 mpxy--3 -Wl- l 0-C(b- 6 -D-gIucNsy-9-Wne(3 -desOxy- l0-hydrxyaioin B ) (C S - 1 2);l, 8, l0- trihydrOxyl-3-methl -l-O- 6-D-glucoPyranosyl -l0-C(O- p-rsgIucoPmpsyI -9-anthrne (Cs-l3).ComPounds CS-3, CS-5, CS-7 wer isOlated frOm thes plat fOr the fch time, COInPOUnscs-8, Cs-9, cs-10, cs-11, cs-12, cs-l3 are novel cOmpounds, aCcording tO the beSt of myknOWedge. Screened with cOnidia of hauIthe mp, the bo of myce1iageednated frOm cOnidia of Prtubo mp was Observd Whe the mixtUr of CS-l l andcs-l2, aS well aS the COmPoUnd cs-7 were Presot. And the mtw of Cs-1l and cs-12shOwed grOWth-inhibitory activity ofendothlial cells and NIH-3T3 fibrobast in the.