Applied Study On Detection Of Plasmodium In Blood Samples By Multiplex Polymerase Chain Reaction

This study aims to establish multiplex PCR for suitable to detect P/asmodiurn (both Pfalciprurn and P vivax) and evaluate the effect in malaria surveillance The blood samples were treated with Normal Salt(N.S),malaria parasites DNA were amplified by the multiplex polymerase chain reaction using two sets of primers derived from the Pf moderately-repetitive DNA sequence pRBK 1-14 and CO III gene ofPv.. A 210 bp special product for Pf and 370 bp for P.v. were amplified by multiplex PCR, no special products be amplified for Re., Pb. and human WBC, Pf and P.v. samples from different areas show the same amplified results, the method can detect parasitemia level as low as 5.5 X iO~ for Pf.and 1.5 X 106 for Pv.. A total of 146 fever samples were detected ,the sensitivity and specificity were 100% and 98.7% for Pv., 97.9% and 98.7% for Pf. The result of malaria surveillance in southern Fujian shows that multiplex PCR assay is sensitive ,specific and prompt, and can be used for malaria molecular epidemiological, surveillance, evaluation the effect of control measures, diagnosis and determination of malaria species.Postgraduate Zhang Shanying (Pathobiology) Directed by Professor Lu Huimin...