The Experimental And Clinic Study Of Taxol Effect On Lymphocyte

Background: The chemotherapy is one of the important methods to treat malignant tumor, but chemotherapy agent can affect immunological function and influence prognosis of patients when killing the tumor cells. There were some different reports about how the chemotherapy agents affect immunological function. Some scholars thought that chemotherapy can restrain immunological function, and someone thought that chemotherapy can enhance immunological function, and the others thought that the effect was related to cycles of chemotheraphy. Taxol is a new anti-cancer agent in treating ovaries cancer breast cancer and non small cell lung cancer, and it has been widely used. There are some different conclusions on taxol抯 affecting lymphocyte quantity and function in vitro. The cytokine can increase proliferation and killing activity of lymphocyte to enhance immunological function in vivo and in vitro, but it is lack of the study how the cytokine influence the lymphocyte affected by chemotherapy agents. Objective: (1) To investigate the effects of taxol on the quantity and function of lymphocyte in vivo and vitro. (2) To investigate the mechanism of taxol affacting lymphocyte immunological function. (3) To investigate the ectogenesis IL-2 and IFN- a influence on the lymphocyte affected by taxol. Methods: After in cubated with taxol or taxol and cytokine, the percentage of CD3.. CD4.~ CD8~ CD16~ CD19 CD25 and intracellular IL-2 .. IFN v of T lymphocyte were performed by using flow cytometry; The proliferation index of lymphocyte was detected by using MIT method; The neutral glycosphingolipid of lymphocytes membrane were determined by using high performance thin layer chromatography (HIPTLC). The expression rates of CD3 CD4.. CD8.. CDl6, CDI9.. CD25 and intracellular IL-2 IFN- of T lymphocyte in patients before and after chemotherapy with taxol and cisplatin were obtained by using flow cytometry. Results: (1) The expression of CDI6.. CD25 and intracellular IL-2 IFN- y of T lymphocyte decreased after in cubated with taxol in vitro; the proliferation index of lymphocyte also declined; the apoptosis rates of lymphocytes increased; the neutral glycosphingolipid CMH of lymphocytes membrane increased, and GLOB declined. (2) The expression of CD16, CD25 of cultivate lymphocyte and intracellular IL-2 .. IFN- v of I lymphocyte in vitro had no significant changes in the group using taxol contrast to the group using taxol and cytokine, so did the proliferation index .. apoptosis rate and the CMH and GLOB of lymphocytes. (3) The expression of CD3 CD4. CD4/CD8 CD 16., CD25 and intracellular IL-2 IFN- of T lymphocyte in patients declined, while the expression ofCDl9 in patients increased; the immunological index had no significant change among different kinds of disease. The level of CD3, CD4, CD4/CD8, CD16, CD25 and intracellular IL-2 or IFN- , of I lymphocyte changes increased with the cycles of chemotherapy increasing. Conclusion: 1 The taxol can inhibit the cellular immunological function in vitro, and the possible ways may be (I) Taxol can restrain the expresstion of IL-2 receptor of lymphocyte surface. (2) Taxol can inhibit the expression of intracellular IL-2 .. WN- i?of I lymphocyte. (3) Taxol can induce apoptosis of lymphocyte. (4) Taxol can affact the metabolism of the neutral glycosphingolipid of lymphocyte, and make the fraction change of the neutral glycosphingolipid. 2 The ectogenesis IL-2 and I...