Evaluation On The Interrelationship Between A Noval Molecul PTA1(CD226) And Allograft Actue Rejection In Reneal Transplantaion

It is important to distinguish the allograft actue rejection in clinic . Generally , the best way to distinguish and monitor the episodes of allograft acute rejection is biopsy . But this way is invasive, bears the risk of potentially dangerous complication, costly and not being easily accepted by patients. Therefore, the availability of a sensitive, specifiable, non-invasive screening for the detection of allograft acute rejection would be highly desireabl to facilitate the task in clinic. Platelet and T cell activation antigen 1 ( PTA1 , CD226 ) is a novel immuno activated molecul that being clonedsuccessfully in 1997, like the other membran molecul PTA1 have two form of solubl and membranous .To study the interrelationship between PTA1 and allograft actue rejection in renal transplantation , to make it become the probe to distinguish allograft actue rejection. When five cases of 19 after renal transplant recipiebts were distinguished as allograft actue rejection by histopathologic examination , according to respective condition of recipients take the sample of blood , Solid-phase ligand ELISA method was used to analyzed serum of sPTAl in renal transplant recipients and Flow cytometry was emploied to study the expression of PTA1 on h/mphtic cells. The level of serum sPTAl had increased remarkably , the expression of mPTAl on lymphtic cells had enhanced ,and the change of the level of serum sPTAl occurred earlier than clinic symptom and that in histopathology , it had decreased rapidly after enhancing the treatment of immune .The activation of infiltration T cell in the allograft of renal transplantation was a gradual process . The allograft did not show signs of acute rejection to the clinic symptom and/or histopathology under the microscope until the activation reached to a certain level. PTA1 is one of sensitive , specific , non-invasive , repeatable , cheap and creditable guideline to distinguish and monitor for allograft renal transplant recipients , the result is consistent with histopathological examination.