Experimental Study Of Repairing Segmental Bone Defeats With Autologous Free Periosteum And Bone Marrow And Calcium Phosphate Cement (CPC)

Objective: To investigate the efficacy of combined use of antologous free periosteum-bone marrow-CPC in repairing segmental bone defects. In order to find an effective method to repair segmental bone defects in clinic.Methods: 28 experimental rabbits were randomized into A,B groups, 14 rabbits in a group, bilateral 10 mm segmental bone defects were produced in the radius of rabbits. A group: one side defect was repaired with Free Periosteum - bone marrow - CPC (FPBMC group). The other side was aimed as control group. B group: one side defect was repaired with free periosteum - bone marrow-gelfoam (FPBM group), the other side defect was repaired with silaltic - bone marrow-gelfoam (SBM group). The X - ray examinations were done at 1,2,4,6,8,10,12 week after operation. The Lane - Sandhu evaluated method was used to evaluate the quantity of the callus formed in bone defects which displayed on radiograph. The evaluated results were tested by Wilcoxon test, the samples of pathology were done in 2,4,8,12 week after operation, the area of new bone of samples were analysed by computer, and the results were tested by Newman - Keuls test.Result: the X - ray evaluating results were that the repair capability of FPBMC group is best (P<0.01), FPBM group and SBM group are second, control group is worst. From the analytic results of the new bone area by computer, FPBMC group has the best function of new bone formation in the 4th week (P<0.05), no differences between FPBM group and SBM group (P>0.05), three groups are not difference in 8th week (P>0.05). In the 12th week; The Function of new bone formation in FPBMC group and SBM group are better than that in FPBM group (P<0.01), but no differences between FPBMC groups and SBM group (P>0.05).Conclusion: â‘  The combined transplantation of FPBMC has the significant osteogenic competence. The barricaded function of the periosteum of FPBMC group is similar to that of membrane guided bone regeneration (MGBR), it can prevent the surrounding soft tissue into the bone defect area. The combined transplation of FPBMC not only has osteogenesis and osteoinduction of periosteum and bone marrow, but also support function and osteoconduction of CPC, and osteogenic function of periosteum is added by bone marrow and CPC, it can efficiently repair bone defects and will be an effective method for repair bone defects in low/no weight loading area. â‘¡ It is very important the crevices inferior to membrane are kept in MGBR, membranes must have some intensity or filled materials can support membranes . The low intensity of periosteum can not resist to pressure, if lack of filled materials support, the periosteum would be collapsed and the crevices inferior to membrane be eliminated, and the bone defect area be filled by the surrounding soft tissues, so MGBR can not be taken and the osteogenesis of periosteum will be descended, and the bone defects can not be repaired.