A Study On Kinetics Of Sensory Neuropeptides And Antigen-presenting Cells In Human Dental Pulp Of Carious Teeth

Objective: The article studies the change and the spatial relation between sensory neuropeptides and antigen-presenting cells in human dental pulp of carious teeth in different stages , namely,. the calicitonin gene-related peptide, substance P, pulpal dendritic cells and endothelial cells, so as to provide theoretical basis and experimental evidence for clinical selection of the suitable time and medicine to keep pulp vital and to evaluate the role of sensory neuropeptides and antigen-presenting cells in the immune defense mechanisms of the carious pulp.?Method: Thirty-four third molars are chosen for the experiment, the normal pulp as control and the condition of the pulp tissue is classified into three groups according to clinical diagnosis and the depth of carious lesion. The sensory neuropeptides. and antigen-presenting cells are immunohistochemically examined with CGRP, SP, HLA-DR, anti-rabbit monoclonal antibodies and factor W~related antigen multiclonal antibody.The number of positively stained substance are counted and statistically analyzed. To study the relationship between them, double-staining is also done with two antibodies for the first time. Results:K Normal pulpal tissue and the dental carious pulp groups show sensory neuropeptides and antigen-presenting cells, and exhibit prominent accumulation at the perivascular region and the para-odontoblastic. 2^ The dental carious pulp groups all show CGRP positive fibers, with the development of carious lesion, the numbers of CGRP positive fibers are significant difference in four groups (P<0.05).33,With thedevelopment ofcarlous lesion,the substantial nerve fibersconcentrate on the carlous region subjacent to the pulpal endofcarrous lesion,and the numbers of SP are different(P＜0．05),themaximum ms found In the Intermediate carres,and the deep carlous Issmaller In comparison．4,Wath the development of carlous lesion,the substantnal HLA－DRpositive cells concentrate on the carlous region subjacent to thepulpal endofcarlous lesion,the numbers otIHJA－DRare different(P＜0．05)．There is slight Increase In the supe,flclal canes；but thereIs no slgnnflcant difference．5,The numbers of Vwf－positive vessel Increase and these vessels becomelarger．Slgnlflcant difference Is found In four groups(P＜0．05)．6,Double－inununohlstochemlcal staining shows that the tendency ofco－Increase ofCGRPandHLA－DR－positive cells Is apparent In thecarlous region．We can observe the CGRP contacting HLA－DR－positivecells．The neuropeptldeSPapproaches littleby littletotheendothelial cells and contact It．Conclusions:1,The human normal dental pulppossessesCGRP,SPposltlve fibers andantigen－presenting cells are essential for the Inununedefensemechanisms of the dental pulp．2,The quantltatave changes of positive substance maintain positiverelation with the depth of the carlous lesion．In the early Stsge ofcarlee,these s［lbstancos havv potentialo厂 ilctlvely reepondingl。()carlous－derived stimull,the Intensity of loca且 immune responseIncrease strongly during the carlous process．3,The co,increase of CGRP and HLA－DR cells,SP and vWF"vessel Inresponse to carlous stimulus Indicates an evident local 4interaction. The results suggest that both sensory neuropeptides andantigen-presenting cells may play a role in the immune response ofdental pulp.4 > These substances have potential -of actively responding tocarious-derived stimuli, biological medicine is needed in the earlytreatment of dental caries, because it may help tooth better use itsnatural defense potential to meet the carious antigenic challenges,and to keep pulp vital.