| The Demyelinating disease of the central nervous system (CNS) is one of commonest nervous system diseases. Its etiology and pathogenesis are still unknown to date. The disease is generally recognized as an autoimmune phenomenon mediated by a specific antigen associated with some viruses. It is assumed that the de my el inat i n g disease of CNS resembles other antoimmune diseases in the illness process, and all the autoanti gens, unspecific T cell activation and inflammatory reaction are relevant to the disease. Myelin basic protein (MBP) is not only the important element of nervous myelin, but also the major antigen of the demy el i nat i n g disease of CNS. The Experimental allergic e nc ephal omy e 1 it i s (EAE) induced by MBP is an autoimmune disease mediated by T cell. It is similar with the human demyelinating disease of CNS in clinical manifestation, immunopathology and i m m un o c h e m i s t r y ; therefore, the experiment can create a typical animal model to study the de m y e 11 i n a t i n g disease of CNS. The method of animal peripheral blood mononuclear cells (PBMC) and dendritic cells (DC) co-culture with MBP in vitro is agood way to study the pathogenesis of EAE. DC is a strongest a n ti ge n- pr e s en t i n g cell (APC) in the body. It has been known that the cells have dual role in immune response and immunologic tolerance, and adjust immune response in the body. The dual role in the illness process of autoimmune disease has been emphasized and the dual role of p atho ge nic ity and restorativeness has been found, too. The function and differentiation of DC can be changed and orientated through the adjustment of the environment of the cell culture in vitro, and the immune function of DC has the characters of diversity and lability. The study of DC seems to be a breakthrough way to resolve autoimmune disease problem; however, the research on the role of DC in the demyelinating disease of CNS has just started world wide in recent years. There are no any research articles on the role of DC in EAE that have been published in China.This experiment made use of a cell culture method to separate PBMC and DC from BALB/c mice susceptible to EAE and co-culture them with MBP in vitro. The observation of the level change ofinterferon-y(IFN-Y) and nitric oxide(NO) have been made to demonstrate the role of unspecific T cell activation in the demyelinating disease of CNS. To detect the expression of CDllc, a marker antigen of DC1, among PBMC and DC activated by MBP and observe the differentiation of precursor DC toward DC1. For thepurpose of the establishment of the rationale of devising the oriental differentiation of precursor DC or changing the function of DC, promoting the recovery of the disease or reducing the relapse of illness, we have made a experimental foundation.The main results of this experiment are as follows: The level of IFN- Y in PBMC stimulated by MBP increase obviously. The level of the test group is 43.83+6.06 pg/ml, the control group is 28.52 ?2.18 pg/m, and the difference is significant (p < 0 . 0 1 ). (2) T h e level of NO in PBMC stimulation by MBP raise evidently. The levels of the test group and the control group are 180.76 +6.06 umol/L and 95.61+13.09 umol/L respectively, and the difference is considerable (j7<0.01). (3) The expression of CDllc in PBMC stimulated by MBP is evident, the mean fluorescence intensity of CDllc in the test group and the control group are separately 15.16+1.92 D and 8.76+1.70 D, and the difference possesses certain significance (p<0 .0 1 ). The expression of CDllc in DC stimulated by MBP increase obviously, the mean fluorescent intensity of CDllc in the test group and the control group are 17.41+1.62 D and 9.13+0.66 D respectively, and the difference is important (p<0.01). (5) The expression of CDllc in mononuclear cells stimulated by MBP not including DC has no any visible changes, the mean fluorescent intensity of CDllc in the test group is 8.86+1.88 D and in the control group8.69+1.12 D. The difference has no signific... |
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