A Study On The Adhesion Of Human PMN To Endothelial Cells During Cerbral Infarction

Objectives To investigate the adhesion of endothelial cells (EC) to polymorphonuclear leucocytes (PMN) from patients at different phase of cerebral infarction (CI) and the adhesion of PMN to hypoxia/ reoxygenation(H/R)-stimulated endothelial cells. Furthemore, to define the effects of antibodies to intercellular adhesion molecular-l(ICAM-l monolonal antibodies, anti-CD54 antibodies) upon the adhesion. Methods We compared PMN interaction with human umbilical vein endothelial celIs-304(HUVEC-304) with and without anti-CD54 antibodies. Then we compared PMN interaction with H/R-stimulated endothelial cells with and without anti-CD54 antibodies. Results 1 , Adhesion of PMN from normal people to VEC-304 was reduced by Anti-CD54 antibodies (P<0. 05) . 2, Adhesion of PMN from patients of CI within 1 week to VEC-304 increased evidently (P <0.01) , but adhesion of PMN from patients with CI at third week to VEC-304 became normal ( P , 0.05 ) . 3 , Anti-CD54 antibodies reduced adhesion of PMN from patients with CI within 1 week to VEC-304 increased highly ( P < 0.01 ) , but anti-CD54 antibodies reduced adhesion of PMN from patients with CI at third week to VEC-304 slightly ( P < 0.05 ) . 4 , Compared with NonH/R-stimulated endothelial cells , adhesion of PMN from patients with CI to H/R-stimulated VEC-304 increased highly (P<0.01) , and adhesion of PMN to H/R -stimulated VEC-304 was 4 times than adhesion of PMN from normal people. 5 , Adhesion of PMN from patients with CI within 1 week to H/R-stimulated VEC-304 was reduced to 45.20±3.04% from 79.4 + 2.93% by anti-CD54 antibodies, and adhesion of PMN from patients with CI within 1 week to Non-H/R-stimulated VEC-304 was reduced to 27.80 +2.64% from 41.53 +3.09% by anti-CD54 antibodies. 6. Hyperlipid did not activated PMN from patients with CI within 1 week, but hypertention and diabetes enhanced activation of PMN.Conclusion 1 . PMN is involved in cerebral injury after cerebral infarction. 2. Adhesion of PMN to endothelial cells can be mediated by ICAM-1 (CD54), anti-CD54 antibodies can partially block the adhesion. 3 , Hypertention and diabetes have effects on activating PMN .