IGF-I,IGFBP-3,GH And INS In Umbilical Cord Serum: Correlation With Fetal Growth

ObjectiveThe size of human infant at birth depends on the duration of gestation and the rate of fetal growth. However despite extensive investigation, it has been difficult to identify any specific endocrine mechanism which plays an essential role in fetal growth. Recently attention has therefore focused on the autocrine and paracrine actions of IGF and IGFBP. We studied the relationship between IGF-U IGFBP-3, GH, INS and growth parameters to discuss the endocrine factors which affected the fetal growth by assaying the levels of IGF-I, IGFBP-3, GH^ INS in umbilical cord serum. MethodsThe neonat.es were divided into AGA, LGA and SGA according the relationship between birth weight and gestation. The length, birth weight, biparietal diameter, foot length and placental weight of neonates were measured and at same time the levels of IGF-1 and IGFBP-3 were assayed by IRA, level of GH by Immulite and level of INS by RIA. We analyzed the data by SPSS 10. 0 for windows. Results1. There were 146 neonates, including 41 LGA, 79 AGA and 26 SGA. There were significantly statistical differences in length, BW, BPD, FL andPW (all P<0. 001). The growth parameters of AGA were all lower than those of LGA (P<0. 001) and were all higher than those of SGA (p<0. 001).2. The levels of IGF-I and IGFBP-3 in AGA were lower than those of LGA (p<0. OOland P<0. 05) and higher than those of SGA (P<0. 01 and P<0. 001). The level of GH in AGA was lower than that of SGA (P<0. 01). There was no difference between AGA and LGA (P>0.05). The level of INS in AGA was highest. There was significantly statistically difference between AGA and SGA and LGA (P<0.05 and P<0.01).3. There were no differences in IGF-I, IGFBP-3, GH and INS between male and female neonates.4. There was positive correlation between growth parameters and IGF-I and IGFBP-3(all P<0.001).There was negative correlation between length and BW and GH (all P<0. 05)There was no correlation between INS and growth parameters (P>0. 05).5. The levels of IGF-I and IGFBP-3 in AGA and LGA were significantly positive correlation (P<0.01 and P<0. 001), but the correlation was not seen in SGA. IGF-I and IGFBP-3, INS were positive correlation (P<0. 001 and P<0. 01). IGF-I and GH were negative correlation (P<0. 01) in all neonates.6. Five growth parameters and IGF-I were significant correlation in multiple regression analysis.Conclusion1. Length, Bff, BPD, FL and PW could be used to evaluate the growth of human fetus.2. The levels of IGF-I and IGFBP-3 in AGA were higher than those of SGA and lower than those of LGA. But GH and INS were not like that.63. There were no differences in IGF-K IGFBP-3, GH and INS between male and female neonates.4. The IGF-I and IGFBP-3 were correlated to growth parameters closely, especially IGF-I. They might be the indicators for growth of human fetus. But the growth of human fetus was a result which was affected by many factors. The regulating model would be changed by some pathological factors and affected the fetal growth.