ExperimentStudyonReversingtheDegenerationIntervertebralDisc Part Ⅲ: The Constructing Of The Model Of The Adult Degeneration Disc Cells And The Study Of The Expression Of The Adult Degeneration Cells Cultured In Vitro Transfected By Ad/CMV-hTGF-β1

Objectives: To find an appreciate method of culturing adult degeneration cells, Ad/CMV-hTGF-pl was used to transfect the adult degeneration cells and the expression of hTGF-pl and the synthesis of proteoglycan and collagen were studied as well as the ultrastructure was observed. Methods: 1. Organization lump method combined with enzyme assimilation method were used for the primary culture of adult degeneration cells. 2. Ad/CMV-hTGF-pl constructed in our laboratory was used to transfect the adult degeneration cells and the expression of hTGF-pl of the cells of nuclei pulposus and annulus fibrous was studied respectively. 3. The synthesis of proteoglycan and collagen of the transfected adult degeneration cells were studied. 4. The ultrastructure of the transfected cells was observed. Results: 1. A new method was found to culture the adult degeneration cells in vitro . 1. The adult degeneration cells were transfected by Ad/CMV-hTGF-pl successfully, the expression of hTGF-pl and proteoglycan and collagen were higher than the controls all the times, p<0. 05 . 3. The structure and the function of the adult degeneration cells transfected by Ad/CMV-hTGF-pl were improved. Conclusion: 1. The method of combining Organize lump methodwith enzyme assimilation method was appreciate for the culture of the adult degeneration cells in vitro. 1. Ad/CMV-hTGF-pl constructed in our laboratory was effectively in transfecting the adult degeneration cells cultured in vitro, and hTGF-pl can be expressed effectively, the synthesis of proteoglycan and collagen can be improved, the structure and the function of the cells can be improved at the same time.