Study On PTEN/MMAC1/TEP1 Gene Mutation In Gastric Carcinomas

Objective To study the mutation frequency of exon 5 and exon 8 of PTEN/MMAC1/TEP1 gene in gastric carcinomas and investigate the relationship between the gene mutation and the pathological parameters (pathological differentiation and clinical stage) in genesis of gastric carcinomas.Methods Mutation of the exon 5 and exon 8 of PTEN/MMAC1/TEP1 gene was detected in 42 gastric carcinoma samples and matched adjacent normal gastric mucosae with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method. PCR products of mutant samples were analysed by DNA sequencing technique.Result 3 samples with PTEN mutation were found in 42 gastric carcinoma tissues. The mutation rate of the exon 5 and exon 8 of PTEN/MMAC1/ TEP1 gene was 7. 14%. No mutation was found in 42 adjacent normal tissues. The mutation rate of PTEN gene in poorly differentiated group and in well-differentiated group samples was 12% and 0, respectively . The difference was not significant statistically (P>0. 05). Mutation rate of PTEN gene in clinical stage I , II and clinical stage III IV was 5. 88% and 8%, respectively . The difference was also not significant (P>0. 05).Conclusion PTEN gene mutation can be detected in gastric carcinoma tissues and is not found in adjacent normal tissues. PTEN/MMAC1/TEP1 gene mutation might play an role in the genesis of gastric carcinomas. The mutation rate was not related to pathological differentiation and clinical stage.