| Astrocytoma originate from the astrocyte of white matter and gray matter in central nervous system. It is the most general type in glioma, with low cure rate, high relapse rate and bad prognosis. Tumor all manifest high angiogenic proliferation, but new angiogenesis often correlates with biological invasionx malignant degree and clinical relapse of tumor. Tumor angiogenesis is important in the process of tumor occurrence, expansion and metastasis. The volume of the tumor which hasn't angiogenesis is hardly bigger than 1mm3 ~ 2mm3, when angiogenesis happens, tumor grows rapidly. The volume of tumor will increase by 16000?9000 times. New angiogenesis is achieved bymany vascular factor, vascular growth factor, VEGF and NOS take a key role in it. It is popular to study the molecular events in brain tumor, Anti-angiogenesis therapy is a new and promising way to conquer tumor. Recently there are some reports about tumor microvessel density, pathology stage, metastasis and prognosis . The reports about the expression of endothelial nitric oxide synthase(eNOS) in astrocytoma are few in oversea, There isn't in China yet. The reports about the relaptionship between eNOS and MVD are less. The mutual action mechanism between VEGF and NOS is unknown. In the present study, we detected the expression of VEGF, eNOS and measured MVD by immunhistochemistry method in 65 samples of brain astrocytoma, which were obtained from 65 patients who underwent surgery, to investigate the relationship among VEGF, eNOS and MVD. The purpose of this present study is to observe the relationship among VEGF, eNOS and MVD. Meanwhile it is to investigate whether they are different with grade of astrocytoma. Thus, useful information can be supplied for us to deepen the understanding of mechanism of tumor angiogenesis. It is useful to seek potential target for anti-angiogenesis therapy.Materials and methods: Tissues of brain astrocytoma were obtained from 65 patiants who underwent surgical operation. There were 43 males and 22 females. The age of patients ranged from 16 to 70 years old (mean:39.3 years old). 57 samples were supratentorial astrocytoma, 8 samples were located under the tentorium of cerebellum. Dimension of the smallest tumor was 1.0cmx2.0cmx1.0cm, the biggesttumor was 4.0cmx5.0cmx6.0cm. Tumors were graded according to the criterion described by kernohan, Grade I , 24 samples; Grade II, 18 samples; Grade III~IV, 23 samples. A total of 8 samples normal brain tissue were used as normal controls which were obtained from patient who underwent craniotomy decompression. The age of patients ranged from 16 to 70 years old. All samples had been proved by means of pathological diagnosis. All samples divide into four groups. N: Nomal brain tissue group. A1: Grade I of astrocytoma. A2: Grade II of astrocytoma. A3: Grade III~ IV of astrocytoma.Using the polyclonal antibodies of anti-VEGF, anti-eNOS, anti-VWF performed immunohistochemical staining (SABC method). The immunohistochemical staining of eNOS, VEGF was assessed according to this criterion: Those having positive staining in less than 10% of tumor cells were graded as "negative", more than 10% as "positive". MVD was assessed by the means of blood vessel quantity of five 'hot spot' under 400x multiple lens. Statistical analysis was executed by SPSS 10.0 software. P values less than 0.05 were considered significant.Result: (1) The expressions of eNOS^ VEGF in nomal controls are all negative. (2) The positive rate of eNOS in astrocytoma is 60% (39/65). VEGF is 63.1 % (41/65). There are significant difference compared to the nomal controls. (3) The positive rate of eNOS and VEGF in astrocytoma increase along with tumor malignancy degree rise, namely A1 group |
PDF Full Text Request