Study On Preparation And Properties Of PDLLA/HA Composite Scaffold In Tissue Engineering

Poly(lactic acid)(PLA) has been widely used as scaffolds for tissue engineering because of its good processability, good biocompatibility and suitable mechanical properties. But its catabolite would often induce erythrophlogosis.The preparation and properties of the PDLLA/HA compound fiber and cell culture on the PDLLA/HA unwoven meshes had research researched in this paper.PDLLA/HA composite fibers were produced by melt spinning, and the choices of the process parameters such as temperature, revolving speed and drawing speed etc were discussed. It was suitable for melt spinning that temperature was 100~150 ℃, revolving speed was 10~15r/min and drawing speed was 2~5m/min.The influence of the content of HA, molecular weight of PDLLA and the diameter of fibers on the mechanical properties of PDLLA/HA composite fiber had been studied through mechanical tests. The tensile strength of composite fiber could reach 767.06MPa and the tensile modulus could reach 0.27GPa when molecular weight of PDLLA was 1.529×105, content of HA was 10% and the diamter of fibers was 30~40μm.The changes in molecular weight, mass, mechanical strength, microstructure of fibers and pH value of the buffer solution with time in vitro had been studied. The study shows that degradation mechanism of PDLLA/HA compound fiber was indicative of an autocatalysis process and simple noumenon hydrolysis. It presentes the modal of "bimorph degradation".Unwoven meshes were formed by PDLLA/HA composite fibers with diameter of 10~ 15μm and embedded by I collagen albumen, polylysine and collagen albumen of the rats' tails. It was found that these three different embedded scaffolds could promote adhesion, proliferation, differentiation and matrix secretion of the cells. There was no obvious difference among them, but all of them were more excellent than unembedded one.