Changes Of TrkB And Ras-MAPK In Role Of BDNF For Hypoxia-induced Embryonic Cortical Neurons

Brain-derived neurotrophic factor (BDNF) may play an important role against neurotoxicity. We investigated the changes of TrkB and Ras-MAPK in role of BDNF for neurotoxicity of hypoxia- induced neurons from primary cultures of embryonic rat cerebella cortical in this study.Objective:Observe the changes of TrkB and Ras-MAPK in role of brain derived neurotrophic factor (BDNF) for neurotoxicity of hypoxia-induced embryonic rat cerebella cortical neurons.Methods:Detect intracellular levels of TrkB,phosphated TrkB tyrosine and MARK in cultured embryonic rat cerebella cortical in different time groups of hypoxia using Immunofluorescence and laser scanning confocal microscope,and half- quantitative analysis was done to explore the changes of them.Results:The levels of TrkB and phosphated TrkB tyrosine and MARK in plasma were significantly increased and the levels of MAPK in nucleus were significantly increased too in hypoxia embryonic cerebella cortical neurons in BDNF groups comparing to the control group without BDNF.Conclusion:The Ras-MAPK approach,probably,was the major signal transferring way of BDNF in protecting the cortical neurons from hypoxia-induced neurotoxicity. The approach of signal transferring began with tyrosine phosphorylation of TrkB receptors and MAPK was the key substance.