Effect Of Reorganized-human Growth Hormone On Cell Cycle Cinetics In Liver Cancer In Vitro

Objectives To study the effect of reorganized-human growth hormone (r-hGH) on cycle kinetics and apoptosis of liver cancer cell line, 7402 cells. Methods Liver cancer cells (7402 cells) were cultured for 24 hours with r-hGH at different degrees of concentration with or without DDR Cells undergoing apoptosis and differentiation were determined by flow cytometry(FCM). Results By comparision of the results between culturning with and without r-hGH. percentage of cells in Go~Gi phase dropped (P< 0.05), percentage of cells in S phase increased (P<0.05). Addition of r-hGH and DDP to the culture medium increases the percentage of apoptosis of liver cancer cells comparing with those adding DDP only (P<0.05). Conclusions Liver cancer cells(7402 cells) might have expression for the hGH receptor. In vitro r-hGH might induce differentiation of liver cancer cells, stimulate the combination of DNA and reduce the cells in Go~Gi phase, it then might improve the sensibility of reaction of tumor to the special-staged chemical treatment . Chemotherapy together with r-hGH may increase apoptosis of liver cancer cells.