Study Of Effect On Fufangbiejiaruanganfang Drug Serum On Hepatic Stellate Cell In Vitro

Hepatic fibrosis is a response to chronic liver injury, which is the outcome of deposition of hepatic fibrosis tissue. The mechanism of hepatic fibrosis is the imbalance of synthesis and degradation of extracellular matrix. The progress of hepatic fibrosis is sclerosis. There are pseudo leaflets in hepatic veins and the common structure is destructed. cirrhosis of liver can lead to hepatic malfunction. So active treatment of hepatic fibrosis can postpone and block formation of cirrhosis of liver, sometimes it can inversio the process of hepatic.Recently, lots of data indicate HSC is the key cell of formation of ECM and HSC is also the core link of occurrenc of hepatic fibrosis. Activation of HSC is the main step of formation of collagen. Change in character and function of cell arise after the activation of HSC. The mostly manifestation is the imbalance of synthesis and degradation of extracellular matrix. Activation of HSC is made up of start of activation and continue of activation. And it is closely related to signal pathway in cell and regulation of nuclear transcription factor. The measure in aim of HSC include: ①remove the etiological factor, relieve the hepatic inflammation. ②selectivity prevent the formation of ECM by HSC. ③Facilitate decomposition of ECM by HSC. ④Facilitate apoptosis of excess HSC.At present western medical doctor are concentrate on the some aspects about treatment of hepatic fibrosis which include prevention of hepatic injury; intervention of signal conduct pathway in cell ; decrease the formation of collagen; increase the degradation of collagen; facilitate the apoptosis of HSC.The law of tradition medicine on hepatic fibrosis include huoxuehuayu;ruangansanjie;qingrelishi;tiaolipiwei et al. Our study investigate the mechanism of treatment of hepatic fibrosis by fufangbiejiaruanganfang. The content of fufangbiejiaruanganfang include biejia; dangseng; et al. The aim of study is to know the influence on proliferation of HSC; activation of HSC; synthesis and degradation of extracellular matrix; signal conduct pathway; nuclear transcript factor.1.the study of effect on proliferation of HSC by fufangbiejiaruanganfangIn view of in vitro,our experiment observe the effect of proliferation ofHSC by influence of different serum. The result indicate that hepatic fibrosis is a common chronic hepatic disease. The two main points of hepatic fibrosis are proliferation of HSC and deposition of ECM. Proliferation of HSC can lead to synthesis of ECM including collagen and proteoglycan and so on. So proliferation of HSC is closely related to occurrence and progress of hepatic fibrosis. Our experiment indicate that different doze of fufangbiejiaruanganfang drug serum can decrease the proliferation of HSC. And high doze and medium doze have more effective result. So it may be the effect of fufangbiejiaruanganfang drug serum is depend on the concentration of fufangbiejiaruanganfang doze. The mechanism of treatment may be related to the content in fufangbiejiaruanganfang drug serum can block the excretion of PDGF by HSC and binding of PDGF and receptor of PDGF. So the mechanism of fufangbiejiaruanganfang may be related to decreasing the proliferation of HSC.2．the study of effect on activation of HSC by fufangbiejiaruanganfangOur experiment on effect on expression of α-SMA,desmin,synaptophysin by technology of immunohistochemistry. Our finding indicates that the main point of hepatic fibrosis is activation of HSC. Expression of desmin,synaptophysin is earlier than expression of α-SMA. The outcome of experiment indicates that fufangbiejiaruangajfang drug serum can decrease the amount and area of desmin,synaptophysin andα-SMA in different time. And we estimate the mechanism of effect on expression of α-SMA,desmin,synaptophysin may be different. More investigation should be done. 3. the study of effect on balance of synthesis and degradation of extracellular matrix by fufangbiejiaruanganfangActivatived HSC is the main source of excessive ECM in hepatic fibros...