The Influence Of Cytokines On The State Of An Illness, Replication Of HBV And Occurrence Of HBVDNA BCP Mutation In Patients With Chronic Hepatitis B

Objective: To probe into the influence IL-2, IFN-r, IL-4 and IL-10 on the state of an illness, replication of HBV and occurrence of HBVDNA BCP mutation in patients with chronic hepatitis B.Methods: Microplate hybridization -ELISA and Fluorescence Quantitative PCR (FQ-PCR) technology were applied to detect HBVDNA BCP mutation and HBVDNA quantity respectively. The level of IL-2, IFN-r, IL-4 and IL-10 in sera was investigated by ELISA method.Results: (1) The results shown that the level of IL-2, IFN-r, IL-4 and IL-10 in the groups of chronic heavy hepatitis B(CHH), chronic hepatitis B ( including light, mediate degreed ) (CH) and liver cirrhosis (LC) was 87. 8±16. 9, 265.8± 228.2, 77.7±95.0,109.8±48.4, 58. 5±20. 5, 73.6±52.6, 66.9±51.8, 77. 2±33.7 and 52.4±20.3, 32.3±27.5, 50.2±35.3, 56.9±34.5 respectively; The level of IL-2, IFN-r and IL-10 in CHH group was significant higher than those in CH and LC groups (P<0. 05), but no significant difference was seen in the leverl of IL-4 (P>0. 05). The level of IL-2, IFN-r, IL-4 and IL-10 in the died and survival patients was 91.0 ± 19. 9, 280.7 ± 243.9, 86.8±101.6, 70. 2 ± 49. 6 and 66. 7 ± 19.0, 93.9 ± 75.9, 61.9 ± 50.6, 75. 1 ± 31.6 respectively. The analysis result showed significant difference between the two groups in the level of IL-2 and IFN-r (P<0. 05), but no significant difference was seen in the level of IL-4 and IL-10 (P>0. 05). (2) The level of IL-2, IFN-r, IL-4 and IL-10 in the HBVDNA BCP mutation and no-mutation groups was 61.4 ± 24.7, 128.3 ± 107.1, 66.3 ± 56.7, 76. 7 ± 54. 5 and 67. 1±24. 3, 93.1±98.7, 59.4 ± 43.3, 72. 5 ± 37. 2 respectively, the matched analysis results showed no significant difference between the two groups (P>0. 1). (3) Thequantity of HBVDNA in sera of patients with chronic hepatitis B was lower with the elevation of the IL-2 level, and showed significant relationship (r=-0. 9722, P<0. 05).Conclusion: (1) The level of IL-2, IFN-r and IL-10 might affect on the development and prognosis of chronic hepatitis B. (2) The immune pressure could be hardly relation to the occurrence of HBVDNA BCP mutation. (3) IL-2 could restrain the replication of hepatitis B virus.