| Objective and Background:Diabetic nephropathy (DN) is one of serious and main microvessel complications of diabetes mellitus. It is also one of main causes that result in ESRD. The feature pathology of DN is progressive renal fibrosis associated with glomemlus lesion and tubulointerstitial fibrosis, which characterized by structural changes such as inflammatory cells infiltrate and proliferate and renal tubules atrophy or expand deformation following accumulating deposition of collagen components within the renal interstitial.Now, more and more attentions are paid to tubulointerstitial lesion during the progressive fibrosis of chronical nephropathy. Some investigations argued interstitial cells, specially, fibroblasts that may play key role during the interstitial fibrosis. Lately, some investigations further consider fibroblasts root in not only self-proliferation, but partly transformed from renal tubular epithelial cells. Furthermore, the TGF- β 1 that is recognized as one of key cytokines during the tubulointerstitial fibrosis may induce the transformation from renal tubular epithelial cells into fibroblast during the progressive interstitial fibrosis besides accelerate the proliferation of fibroblasts and interstitial cells. However, It is still not clear whether the parallel transformation is involved in the fibrosis of DN.In the present study, we observed the expression of TGF- β 1 ,Vimentin and Desmin in the renal cortex of STZ induced diabetic rats by RT-PCR on the different days after the DM rat model established and tried to find out their expressional law and interface.Experiment Design and Methods87 Sprague-Dawley male rats weighing 180-200g were randomly divided into two groups: Diabetes mellitus rats group (DM, n=49) and normal control rats group (NC, n=38). Diabetes was induced in 49 male SD rats by single intraperitoneal injection of 1.0%STZ (70mg/kg). 38 age and weight, sex matched SD control rats intraperitoneal inject same dose of citromalic acid buffer. The levels of blood glucose were determined 48 hours and 72 hours after the injection of STZ. The rats with blood glucose levels ≥ 16.7mmol/l and urine glucose above +++ for 3days were regarded as diabetic rats. Six rats of each group were sacrificed on the 3rd, the 7th, the 14th, the 30th, the 60th and the 90th day after the DM rat model established. Blood samples were collected from femoral arteries for the measurement of blood glucose levels and creatinine levels. Rats were weighed before sacrificed and their urine samples were collected for the measurement of urine albumen and creatinine. After death, kidneys were removed instantly, weighed and dissected to separate the cortices for total RNA isolating. Total RNA of the renal cortex were extracted by trizol reagent. TGF- β1 , Vimentin and Desmin mRNA levels of renal cortex were examined by RT-PCR.Results1. The blood glucose values of DM group were significantly higher than those ofNC group at all observed time points after the DM rat model established (P<0.01) .2. The blood creatinine values of DM group were significantly higher than those of NC group on the 3rd, the 7th, the 14th, the 30th and the 60th day after the DM rat model established (P<0.05or<0.01) except for the 90th day.The urine creatinine values of DM group were significantly lower than those of NC group on the 3rd, the 14th, the 30th and the 60th day after the DM rat model established (P<0.05or<0.01) except for the 7th day.The creatinine clearance rate values of DM group were significantly lower than those of NC group on the 3rd, the 14th, the 30th and the 60th day after the DM rat model established (P<0.05or<0.01) except for the 7th day.3. The values of urine ALB/Cr of DM group were significantly higher than those of NC group at all observed time points after the DM rat model established(P<0.05or <0.01) . There was no significant difference among the observed time points in the DM group (P>0.05) and in the NC group (P>0.05) .4. The kidney weight of DM grou... |
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