The Immunoloregulation Role Of CD2 In Acute Rejection After Small Bowel Transplantation In Rats

Objectives1. To practice makes perfect to establish the models of thesegmental small bowel transplantation in rats.2. To study the relationship between the positive expression rate of CD2 in peripheral blood and the acute rejection, after small bowel transplantation.3. To investigate the theraputic effects of 0X34 in acute rejection after small bowel transplantation (SET) in rats. Methods1. Two species of inbred line rats (SD?Wistar) which were total 72 were randomly pair-matching divided into 4 groups (A?B?C?D) . Group A(n=18) conducted fictitious operation and fed with common forage; Group B(n=18) conducted iso-series SBT and the treatment of post-operation with routine fluid replacement and antibioltic ; Group C(n=18) conducted aniso-series SBT and the treatment was same as group B; Group D (n=18) conducted the same operation as group C , but the treatment were routine fluid replacement?antibioltic and 0X34. Samples of grafts and peripheral blood were taken from 3?5?7 days after SBT. The grafts were stained by HE and the peripheral blood were taken from abdomial aorta under sterile condition. All samples were detected by pathology and flow cytometer.2.0X34 were marked by FITC and measured F/P by ultravioletDept. Ophthalmology FMMUspectrophotometer;. Results1. The survival time of group C was significant Shortening among other three groups(P<0. 05). But there was no significant difference among groupA?B? D(P>0. 05).2. The pathological examination of grafts from group C showed that the drgree of acute rejection was from light to weight when the samples were taken form 3?5? 7 days by turns after SBT. Part of grafts from group D were also occurred light drgree of acute rejection in 5?7 days after SBT while none of group A?B had pathological evidence of acute rejection.3.The positive expression rate of CD2 in peripheral blood of group C were significant increased among other three groups (P<0. 05). But there was no significant difference among groupA?B?D(P>0. 05). In addition, following the time of samples, the positive expression rate of CD2 were descended gradually Conclusions1. Suddenly increasing of the positive expression rate of CD2 fiercely suggested the occurrence of acute rejection after small bowel transplantation,it could be thinked as one of immune markers which can predict the occurrence of acute rejection at an early stage.2. The results demonstrate the pivotal role of CD2 signaling in mediating allogeneic immune reactions after SBT. To block the passway of CD2 signaling in mediating allogeneic immune reactionsDept. Ophthalmology FMMUcan significantly down-modulate the immune reactions after SET.3. 0X34 significantly suppressed acute rejection after SBT in vivo. Duration of CD2 down-modulation by 0X34 was clearly dose dependent and linked to the presence of circulating antibody in blood.It maybe a new way of immunosuppressive therapy after SBT.