Studies On The Quality Standard Of Lingguizhugan Decoction

The extraction process of Lingguizhugan decoction was studied in this paper. Using water and alcohol of different concentrations as the extracting solvent, pharmacological activity with 11 kinds of different extracting methods were studied, 95% alcohol was confirmed as the optimal solvent by pharmacological experiments. The optimal alcohol extraction process was studied by orthogonal design with extractive yield, dehydrotumulosic acid, cinnamic acid and glycyrrhizic acid content as indices. Three factors were selected in this experiment, including the solvent consumption, time of extraction and times of extraction. The result showed that the optimum extracting condition was 95% alcohol consumpted ten times the amount of material, and three times for sixty minutes each time.Cinnamon twig, Atractylodes rhizome and Licorice root in Lingguizhugan decoction were identified by TLC with the dots clear and reproducible. Cinnamon twig was identified with petroleum ether(60-90 )-ethyl acetate(17:3) as developer and dinitrophenylhydrazine ethanol solution as coloration using silica gel G. Atractylodes rhizome was identified with petroleum ether(60-90 )-ethyl acetate(50:1) as developer and 5% vanillin sulfuric acid solution as coloration using silica gel G.: ABSTRACTLicorice root was identified with n-butanol-SmoL-L-1 ammonia solution-ethanol (5:2:1) as developer using silica gel HF254 and observed at 254 nm using ultraviolet lamp.Dehydrotumulosic acid, cinnamic acid and glycyrrhizic acid in Lingguizhugan decoction were determined by RP-HPLC. Dehydrotumulosic acid was analyzed by a Hypersil-Cig column with acetonitrile-water-tetrahydrofurant-acetic acid (120:100:4.5:2,v/v) as the mobile phase and 242nm detection wavelength was selected. The results showed the calibration curve was linear in the range of 9-180 g-mL-1 and correlation coefficient was 0.9998. The average recovery of dehydro-tumulosic acid was 97.8 % (RSD=2.6%) . Cinnamic acid was analyzed by a Hypersil-C)8 column with methanol-acetonitrile-water-acetic acid (10:22:70:1.4,v/v) as the mobile phase, p-dimethylaminobenzaldehyde as the internal standard was used ,and 254nm detection wavelength was selected. The results showed the calibration curve was linear in the range of 8.4-168[ig-mL~' and correlation coefficient was 0.9999. The average recovery of cinnamic acid was 98.2 % (RSD=1.9 %) .Glycyrrhizic acid was analyzed by a Hypersil-Cig column with methanol-acetonitrile-water-acetic acid(l5:35:45:0.9,v/v) as the mobile phase, naphthalene as the internal standard was used, and 254nm detection wavelength was selected. The results showed the calibration curve was linear in the range of 19.4-388 g-mL-1 and correlation coefficient was 0.9998. The average recovery of glycyrrhizic acid was 98.3 % (RSD=2.4 %) . Above methods were simple, rapid, accurate and provided a qualitative and quantitative basis for the quality assessment of Lingguizhugan: ABSTRACTdecoction.Lingguizhugan decoction is composed of 4 kinds of Traditional Chinese Medicine. In this paper the extraction process, qualitative and quantitative of Lingguizhugan decoction were studied and provided basis for the new form of a drug and quality assessment.