| Objective:To study on gene expression profiles of retroperitoneal adipose tissue in high-fat diet induced SD rats and antisense oligodeoxynucleotide treatment SD rats. To investigate the functions of differential expression genes. Methods:The cDNA probes were synthesized from mRNA of adipose tissue in normal group, obese control group or antisense group. Then they were hybridized with three identical cDNA microarray membranes containing 18000 human gene blots. After the membranes were scanned, the differential expression genes were screened and their functions were analyzed by bioinformatics. Results:(1) 532, 154, and 22 genes were found significantly differently expressed over 2, 3, and 5 folds respectively in all the differential expression genes of obese group and normal group. In the differential expression genes of obese group and antisense treatment group, 404, 81and 34 genes were found differently expressed over 2, 3, and 5 folds each. (2) Different clusters of genes involved in the onset and development of high-fat diet obesity. The characteristics of gene expression profile of High-fat diet feeding rats' adipose tissue include adipose tissue remodeling, adipocyte apoptosis deficiency and Ca2+ signal transduction pass activating et al. And the distinguished features of gene expression profile of antisensegene treatment rats' adipose tissue are insulin resistance inhibition andadipocyte apoptosis growing in numbers. (3) The genes related to NPYY5 receptor gene treatment were divided into two clusters by theK-means clustering method. Many known genes and unknown genessimilar in function were clustered by the hierarchical clustering method.Conclusion:The expression profiles of rat's adipose tissue before NPY Y5 receptorgene treatment and after this treatment have special biological featuresrespectively. Clustering analysis is a useful method to suspect thefunctions of unknown genes and new functions of known genes. |
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