| Caries is harm for the health of human. It is infection and transmission disease. Mutans streptococci (MS) is main bacteria causing caries. In the present study, the time of colonization by MS and effect factors in children have been investigated and argued in the prime time. In national, there has not news of effected colonization by SS and MS's factors in children. So to study of colonization of Mutans Streptococci and effect factors in infants would be help of interdiction or delay the colonization and transmission of MS. The hope for containing the caries problem are provided in children.Materials and MethodsOne hundred and eighty children ( age range 6-24 months) from Shenyang city were examined for the colonization of MS and SS. They were divided into six groups according to age. Their mother were also examined and completed questionnaires. They have no system diease and havent taken any antibiotics in two weeks ago. All these exam explorer by one dentist. The saliva samples were transported to the labo-ratory in an anaerobic jar and immediately placed in an anaerobic glove box. Apliquots of undiluted and 1/10 and 1/100 and 1/1000 diluted saliva samples over plated on TYCSB agar plates. The plates were incubated at 37癈 for 48 - hour in an anaerobic glove box( 10% H2,10%CO2,80%N2). Colonies were identified as MS by fermentation test and H2O2test. DP is by SPSS statistical analyses.Results1. Of the 180 infants, 99(55.0% ) had colonization of MS. The prime time of colonization is 8 months, detection rate increased along with the manifold of age and teeth erupted. There were significant differences in the baselin values for six groups (x2 = 17. 40, P < 0. 01).2. The prime time of SS - colonization is 8 months. Of the 180 infants, the 75 children who MS and SS were not detected in infants, the 80 children who only MS were detected, the 6 children who only SS were detected, the 19 children who MS and SS were detected. The children who harbored both MS and SS were earlier detected than only colonization of MS ( P < 0. 01).3. The study showed a correlation between the prime time of colonization and the MS level of his mother.4. The study showed a correlation between the prime time of colonization and feeding mode, breastfeeding, feeding frequency during bedtime, asleep habits.DiscussionMany investigators have reported that MS were not detected before teeth erupted; detection rate increased along with the manifold of age and teeth erupted . The results of the present study support these findings. Using MS or MSB plate, the prime time of colonization is 6 - 13 months. The results of the present study is that the prime time of colonization is 8 months, there were significant differences in the baselin values for six groups(x2 = 17.40,P <0.01).Streptococci sobrinus ( SS) is one of Mantus Streptococci, it is correlation with caries . The results of the present study is that the prime time of SS - colonization is 8 months. Of the 180 infants, the 75 children who MS and SS were not detected in infants, the 80 children who only MS were detected, the 6 children who only SS were detected, the 19 children who MS and SS were detected. The children who harbored both MS and SS were earlier detected than only colonization of MS (P<0.01).Several studies have shown identical MS in mother and child indicating mother is the main source of MS to her child. There is a correlation between the prime time of colonization or caries in child and the MS level of his mother; A correlation between the development of nursing caries and improper feeding habits. The results of the present study is agree with it. The study showed a correlation between the prime time of colonization and feeding mode, breastfeeding, feeding frequency during bedtime, asleep habits.Therefore we take methods that to reduce the level of mother "MS by preventive, to advocate reasonable feeding mode and asleep habits,these would interdice or delay MSs colonization and transmission and decrease caries.Con... |
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