The Expression Of CD105 In Endometrium And The Difference Of CD105, CD34 And VⅢ-RA Expressed

Objective: At present pan-endothelial cell markers, such as CD34 and VIII-RA are often used in the study of angiogenesis. But because of the highly heterogeneity in endothelial cells, those antibody of pan-endothelial cell markers can widely stain with normal vessels. Thus those antibodies lose the specificity to bind the newborn blood vessels in tumor tissues. A new endothelial cell marker, CD105, was used in study of angiogenesis in tumor tissues now, thereby to study the possibility of anti-angiogenesis therapy of tumor. CD105 is the specific membrane receptor of TGF- β1 and TGF- β3, and it highly expresses on the surface of endothelial cells in those tissues with angiogenesis, such as granuloma, embryonic tissues, psoriasis and tumor tissues. CD105 shows satisfactory developing prospect in diagnosis, prognosis and therapy of tumor. In our study, mice anti-human CD105 monoclonal antibody E9 was utilized to detect the angiogenesis in different stage of endometrium by stain with CD105, which highly expressed on the surface of the newborn blood vessels. And we hope to provide the foundation of anti-angiogenesis therapy in tumor. At the same time, we also compared CD105 with CD34 and VIII-RA, so as to study the property of anti-CD 105 monoclonal antibody E9 in binding with actived endothelial cells. Methods: 153 cases fresh of hysterectomy were collected, including proliferative(48), secretory(43), atrophy(21) , simple hyperplasia(29), complex hyperplasia(6), and chronic endometritis(6). The expressions of CD34, CD 105 and VIII-RA in those endometrium tissues were detected by immunohistochemistry. Microvessel density (MVD) was quantitative measured. And we also analyzed the difference of MVD in different stage of endometrium. Result: The MVD of endometrium has significant difference among proliferative phase, secretory phase and simple hyperplasia by detecting CD105 on the surface of endothelial cells (P<0.01) . The MVD of endometrium also has significant difference between chronic endometritis and atrophic endometrium ( P<0.05 ) . The MVD of endometrium separately marked by CD105, CD34 and VIII-RA has significant difference both in proliferative phase and secretory phase ( P<0.01 ) .Conclusions: 1. There was significant difference of MVD between proliferative phase endometrium and secretory phase endometrium. Under the effect of estrogen and progestogen, small vessels of endometrium hyperplasia constantly, and thus, the MVD in secretory phase endometrium are increased, 2. MVD also increased in chronic endometritis, and this suggests the inflammation can activate the endothelial cells, on which CD105 highly expressed. 3. Different endothelial cell markers, such as CD34 and VIII-RA, more easily express on the normal maturity vessels, while CD105 mainly express on the newborn blood vessels. 4. When anti-angiogenesis therapy under clinical experiment,we should choose relative quiescence stage of blood vessels, and it can be weak effect on female reproductive cycle.Postgraduate Chang Hong (Pathology) Directed by Professor Yu Jianxian...