| Objective: To study the correlation of serum selenium level, diet factors, Polymorphism of CYPlAlMspl, CYP2E1 Rsal and GSTM1, independently and in combination with the risk of lung cancer.Methods: A case-control study which include 58 cases of lung cancer and 58 controls was conducted. All subjects were investigated with a uniform questionnaire. Polymorphism of CYPlAlMspl and CYP2E1 Rsal were detected by PCR-RELP and polymorphism of GSTM1 was detected by PCR. The serum selenium level was determined by GHAFS.Results:1. Average serum level of selenium among the lung cancer cases (0.088 + 0.020mg/L) was significantly lower than that among controls (0.102 + 0.023 mg/L), P=0.001. The OR of lung cancer, comparing the lower level ( <0.109 mg/L)with the higher serum level ( ^0.109 mg/L), was 4.44 (95%CI1.63-12.01).2. The results showed that following diet and cooking factors were associated independently with the risk of lung cancer: intake of dairy products (OR 0.31, 95%CI 0.10-1.04), vegetables (OR 0.45, 95%CI 0.08-2.55), carrot (OR 0.24, 95%CI 0.10-0.58), fresh fruits(OR 0.66, 95%CI 0.34-1.29), garlic foods(OR 0.55, 95%CI 0.19-1.59), cooking with animal oil (OR 5.34, 95%CI 1.13-20.16)), intake of fried foods(OR 2.26, 95%CI 0.79-6.40), smoked foods(OR 2.70, 95%CI 0.74-3.91), pot-stewed foods(OR 2.29, 95%CI 0.56-9.33), meat (OR 1.42, 95%CI 0.23-8.84), on using air pump (OR 2.83, 95%CI 1.34-5.98) and air exhaust fan(OR 2.81, 95%CI 1.21-6.52).3. There was no significantly different in frequencies of polymorphism of CYP1A1 Mspl, CYP2ElRsaI and GSTM1 between the two groups (P>0.05). The genotypes were no significantly associated with the risk of lung cancer: CYP1A1 m2m2 (OR 1.53, 95%CI0.65-3.62 ) , CYPlAlm,m2 (OR 0.90, 95%CI 0.37-2.20) , CYPlAlm,m1 (OR 1.00) ; CYP2ElCiC,( OR 1.45 , 95%CI 0.67-3.16 ) CYP2EldC2 (OR 1.00 ) , CYP2E1C2C2 (OR 2.35, 95%CI 0.50-11.07) ; GSTM1 null(0/0) (OR 1.73, 95%CI0.84-3.58) .4. People who had the lower serum selenium and earring CYP1A1 rri2m2 had higher risk of lung cancer(OR9.00, P=0.006) comparing with those who had the higher serum selenium and earring CYPlAlminii. The CYP2ElCiCi with the lower serum selenium level had 9.86-fold risk of lung cancer compared to the CYP2ElC]C2with the higher serum selenium level and there was significant difference (p=0.041). The OR for the combination of GSTM1 null(0/0) genotype and the lower serum selenium level was 5.65(p=0.011).5. Less carrot consumption with the CYP1A1 m2m2 had 10.36-fold (p=0.003) risk of lung cancer compared to more carrot consumption with the CYPlAlminii. Less carrot consumption with the CYP2ElCiCi had 4.76-fold (p=0.020) risk of lung cancer compared to more carrot consumption with the CYP2ElCiC2- The OR for the combination of less carrot consumption and the GSTM1 null(0/0) genotype was 6.30(95%CI1.88-21.05).6. The results of multivariate logistic regression showed that following factors were significant associated with the risk of lung cancer: intake of carrot(OR0.18, 95%CI0.05-0.65), no using air exhaust fan (OR3.93, 95%CI 1.36-11.38), amount of smoking (OR 1.34, 95%CI 1.06-1.69).Conclusion:1.There was significant inverse association between the serum selenium level and the risk of lung cancer.2.Intake of dairy products, vegetables, fresh fruits and garlic foods were inversely but not significantly correlated with the risk of lung cancer. The consumption of carrot had been significant inversely correlated with the risk of lung cancer. Intake of fried foods, smoked foods, pot-stewed foods and meat were negatively but not significantly associated with the risk of lung cancer. There was significant negative association between cooking with animal oil, no using air pump and air exhaust fan.3.NO significant correlation of polymorphism of CYP1A1 Mspl, CYPlElRsal and GSTM1 independently with the risk of lung cancer has been observed.4. The combination of lower serum selenium level with the polymorphism of CYP1A1 Mspl, CYP2ElRsaI and GS... |
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