| Objective To explore the detrimental effects of the compound factors of hot and humid environment and scald injury on the surface of wound and the whole physical responses of rats, and the protective function of immediate cooling therapy (ICT) under the influence of the above compound factors. The study, offering theoretical basis to establish the assessment criteria of ICT on scald injury in the south battlefields and regions, is to lay experiments foundation to the research of cooling dresses adaptable to the emergency treatment of scald in the south battlefields and regions.Methods In section I, eight Wistar rats were divided randomly into two groups with four for each. The rats were scalded by 99 ℃ tap water for three seconds and ten seconds respectively. The eye and histological assessments were employed to IcmX 1cm skin removed before and after scalding. In section II, twenty-four Wistar rats, six for each, were distributed randomly into four groups: normal temperature controlled (NTC), normal temperature cold therapy (NCT), hot and humid controlled (HC), and hot and humid cold therapy (HCT). Different interfering conditions were applied to the superficial second-degree rats scalded models, Tdb (26.33?.29) ℃, rh (71.05?.57) % for two normal temperature groups, Tdb (35.33?.35) ℃, rh (70.81?.38) % for two hot and humid groups, no cooling dresses, being offered to the two cold groups, for the two hot and humid groups. The exposure time of each group was 120 minutes. Tr, Ts, HR, MAP and RF served as observing indexes. During the test, the physiological indexes were recorded once per 20 minutes. In section III, 24 Wistar rats were divided randomly into four groups, normal-controlled (NC), normal-temperature-controlled (NTC), hot and humid-controlled (HC) and hot and humid cold therapy (HCT), six for each. No interference was applied to the first group; scaled models were made in the last three groups. Under the same interfering conditions as that of second section were IcmX 1cm skins removed in the process of scalding and 120 min after.Results In section I, the four rats of the first group were scalded in superficial degree II and the other four of the second group were in deep degree II, judged with the depth of the scalded wound and normal skin accessories reservation. In section II, a conclusion was drawn, based on the comparison of seven times inner-group, inter-group and inter-time assessments, that Tr and Ts (P<0.05) raised with hot and humid factors and decreased when cold therapy was applied to. An interaction, having influence on Tr(P=0.03) was found between temperature and cooling therapy as well as that on Ts (P<0.05) between time and condition temperature(.P<0.002), time and cold therapy(P<0.05), time, condition temperature and cold therapy(.P<0.05). Tr and Ts were reduced (3.14?.87)X: and (1.92?.13) ℃ respectively bycold therapy in normal temperature groups and (0.98?.87) ℃ and (2.36?.03) ℃ in high temperature groups. Tr's difference between HC and HCT is lower than that between NTC and NCT. Ts' difference between HC and HCT is higher than that between NTC and NCT. MAP is not affected by environment temperature and cold therapy. (P>0.05). HR (P=0.003) was higher in high HC than in NTC and NCT, and HR (P=0.002) was lower in HCT than in NTC and HC. The seven inner-group assessments and inter-group comparison showed that cooling therapy did great help in lowering RF (.P<0.05) in hot and humid environment. According to the histological observation of NTC, the pathological changes of section III worsened two hours after scalding, with the expression of further degenerate necrosis of epidermis, skin accessories and blood epithelium and more serious dermis edema. The pathological change of HC was worse than that of NTC and lighter than that of HC.Conclusion (1) The superficial degree II Wistar rats scald model, which is adaptable to the evaluation of cold therapy on scalded wounds and physiological response, is easy to be made under test condition of 99 ℃ tap water an... |
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