Effect Of Xiaotan Sanjie Recipe On Expression Of MMP And TIMP In Gastric Carcinoma

According to Traditional Chinese Medicine, "phlegm" and "blood stasis" is the material basis to produce carcinoma. Jin Long She Oral Liquor, which is made according to Xiaotan Sanjie Recipe, has produced better curative effect on clinic. Based on the previous clinical and experimental research, we study the effect of Xiaotan Sanjie Recipe on expression of cell matrix metalloproteinase(MMP) and tissue inhibitor of metalloproteinase of gastric carcinoma, such as MMP2,TIMP2,MT1-MMP(MMP14). To further investigate mechanism that Xiaotan Sanjie Recipe inhibits invasion and metastasis of gastric carcinomatous cell.Pharmacelltic blood serum of Xiaotan Sanjie Recipe was made, MKN-45 human gastric carcinoma cell was cultivated. We observed cell counting and despite growth curve of the gastric carcinomatous cell which is affected by Pharmacelltic blood serum of Xiaotan Sanjie Recipe; OD value was measured by MTT method; carcinomatous cell colony number on lavor carang was detected. Furthermore, a transplantable tumor model of MKN-45 human gastric carcinoma in nude mice was established, nude mice were fed with Xiaotan Sanjie Recipe. Then the expression of MMP2,TIMP2,MT1-MMP was studied with S-P immunohistochemical method in vitro and in vivo. In addition, we still observed collagen in gastric carcinoma tissue of nude mice by Projection eletron microscope.The following are the main results:1. Cell counting in traditional Chinese medicine group was obviously lower than that in the controlled group, the rate of inhibiting was 32.94%; OD value and cell colony number on lavor carang in traditional Chinese medicine group was also lower than that in the controlled group. All this produces were certificated that Xiaotan Sanjie Recipe could inhibit proliferation and infiltration of gastric carcinomatous cell.2. Expression of MMP2 and MT1-MMP in traditional Chinese medicine group were obviously lower than that of the controlled group in vitro and in vivo, andexpression of TIMP2 in traditional Chinese medicine group was higher than that of the controlled group in vitro and vivo, the result of projection eletron microscope manifest that collagen out of carcinomatoud cell in traditional Chinese medicine group was obviously higher than that in the controlled group. So Xiaotan Sanjie Recipe can lower the expression of MMP2 and MT1-MMP, and higher the expression of TIMP2 in carcinomatous cell, which can urge that normal dynamic equilibrium of MMP and TIMP are recovery. Thereby the degradation of extracellular matrix and basement membrance is reduced, infiltration and metastasis of gastric carcinomatous cell are inhibited.